FlyBase Allele Report: Dmel\bru1[UASp.cFa]

General Information

Symbol

Dmel\bru1^UASp.cFa

Species

D. melanogaster

Name

FlyBase ID

FBal0144449

Feature type

allele

Associated gene

Dmel\bru1

Associated Insertion(s)

Carried in Construct

P{UASp-bru1.F}

Key Links

Transgenic product class

cDNA

(Filardo and Ephrussi, 2003)

wild_type

(Filardo and Ephrussi, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Filardo and Ephrussi, 2003)

wild_type

(Filardo and Ephrussi, 2003)

Mutagen

in vitro construct

(Filardo and Ephrussi, 2003)

Progenitor genotype

Carried in construct

P{UASp-bru1.F}

Cytology

Description

UASp regulatory sequences drive expression of the bru1 cDNA.

(Filardo and Ephrussi, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

bru1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

decreased occurrence of cell division | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

increased occurrence of cell division | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

Phenotype Manifest In

dorsal appendage, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

egg chamber | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

egg chorion, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

embryonic/first instar larval cuticle | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

female germline stem cell | larval stage, with Scer\GAL4^{VP16.nanos.UTR}

(Xin et al., 2013)

female germline stem cell | progressive, with Scer\GAL4^{VP16.nanos.UTR}

(Xin et al., 2013)

micropyle | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

nurse cell, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

nurse cell | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

oocyte | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

ring canal | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

Detailed Description

Statement

Reference

Mis-expression of aret^{Scer\UAS.P\T.cFa} in female germline stem cells (GSCs) and their immediate descendants under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in a progressive decline in GSC numbers per germarium within two weeks after eclosion.

In wild-type animals, all the primordial germ cells (PGCs) carry a single spherical fusome (spectrosome) or two dividing spherical fusomes at late third instar. However, all the Scer\GAL4^{nos.UTR.T:Hsim\VP16}>aret^{Scer\UAS.P\T.cFa}-overexpressing gonads contain PGCs with branched fusomes indicative of precocious cell differentiation.

(Xin et al., 2013)

Ovarioles of aret^{Scer\UAS.P\T.cFa}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} flies have a range of germ cell replication defects: In some cases no oocyte develops and endoreplication of the nurse cells fails. In other cases egg chambers have 31 nurse cells, and one oocyte with 5 ring canals instead of 4. In rare cases (<5%) these egg chambers form with a mislocalised oocyte, or with two oocytes. Fused egg chambers also occasionally form. aret^{Scer\UAS.P\T.cFa}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} flies lay fewer eggs than wild-type. Of the eggs that are laid, approximately 5% are clearly ventralised, as assessed by absence or fusion of dorsal appendages. Eggs also occasionally form a posterior micropyle. About 15% of the resulting embryos have cuticles with a typical posterior group phenotype.

(Filardo and Ephrussi, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, bru1^UASp.cFa has increased occurrence of cell division | oogenesis phenotype, non-suppressible by vas^αTub67C.GFP/Scer\GAL4^{VP16.nanos.UTR}

(Filardo and Ephrussi, 2003)

Scer\GAL4^{VP16.nanos.UTR}, bru1^UASp.cFa has decreased occurrence of cell division | oogenesis phenotype, non-suppressible by vas^αTub67C.GFP/Scer\GAL4^{VP16.nanos.UTR}