hh⁴/hh¹¹ ovarioles grown at 28^oC display severe germ-line defects. About 1/5 of ovarioles show obvious germ-line defects. Germ cells appear to differentiate precociously and become necrotic at very high frequency. In addition, somatic cells show under-proliferation defects.

Strong allele.

When hh⁴/hh¹¹ females are grown at 18^oC and raised to 28-29^oC for 6-8 days, compound egg chambers are produced which result from the failure to encapsulate the germline cycts by the somatic follicle cells. Region 1 of the germarium appears normal but region 2 contains round germ-line cells amongst which individual cysts are difficult to distinguish. In the most extremely affected ovarioles a small germarium is attached to a giant chamber which contains all the cysts that would normally be separated into individual egg chambers. These effects are similar to those caused by neurogenic mutants. In addition, follicle cells in young egg chambers sometimes show an increased number of mitotic figures.

Double mutants of wg^hs.PN and hh¹¹ show a similar but not identical phenotype to wg^hs.PN alone, i.e. naked throughout most of the ventral cuticle but with dispersed patches of non-polarized denticles evident at the lateral sides.

Mutant embryos have exclusively row-5-type denticles arranged in a disorganized pattern of ventral midline whorls, with no naked cuticle.

40% length of wild type larvae and exhibit a ventral lawn of denticles associated with the loss of naked cuticle from the ventral surface.

Strong phenotype. Mutant embryos show no obvious segmentation, and are 40% the length of wild type; denticles form a lawn arranged in a number of whorls on the ventral surface as a result of loss of naked cuticle.