86% of mutant embryos lack eve expressing pericardial cells (EPC), the rest show one EPC per hemisegment (instead of the two seen in wild-type). DA1 muscles form as in wild-type. A halving is also seen in the number of myocardial cells seen in segments A2 to A7, though the number seen in segments T3 to A1 remain normal.
In mutant embryos, the ganglion mother cells, GMC1-1a and GMC4-2a fail to divide into their normal progeny. The non-dividing GMC1-1a and GMC4-2a cells are larger than normal and sit where its offspring should normally be. A loss of approximately 10% of EL neurons is also seen.
In mutant embryos the division of neurons are affected in a lineage specific manner. The ganglion mother cell (GMC) of the RP2/sib lineage fails to divide, and assumes the identity of the RP2 neuron. The GMC of the aCC/pCC lineage is similarly affected; it fails to divide and assumes the identity of the aCC. These phenotypes are fully penetrant. Timing and axonal projections from these neurons continue as if they were wildtype. Other lineages are also affected in mutant embryos. The CQ lineage fails to divide in about 50% of hemisegments and the U lineage fails to divide in 65% of hemisegments. The effect on the EL line is minimal.