Egg chambers of females containing homozygous germ line clones develop normally. Eggs derived from these females and fertilised by wild-type sperm develop but die during late embryogenesis (most embryos are arrested by stage 16). Syncytial nuclear cleavage cycles appear normal in these embryos. Later embryos show poor cuticle development and variable segmentation defects consisting of loss and fusion of denticle belts. Defects in the organisation of specific neuromeric segments of the ventral nerve cord are seen at stage 13, especially between segments A4 and A8, which give rise to nerve cord gaps in later stages. The ISN and SNb nerves often fail to join when exiting the central nervous system (CNS). Homozygous embryos (lacking zygotic function) display late defects in the CNS at stage 15 after the CNS begins to condense, showing disarrayed SNb and ISN nerve routes without any nerve cord abnormalities.

Homozygous embryos show little movement within the egg case, fail to hatch and die. The SNb and ISN nerve branches appear disarrayed and the central nervous system does not condense completely.