FlyBase Allele Report: Dmel\CG7483[19]

General Information

Symbol

Dmel\CG7483¹⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0010180

Feature type

allele

Associated gene

Dmel\CG7483

Associated Insertion(s)

Carried in Construct

Also Known As

eIF4AIII¹⁹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G150S.

(Palacios et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:8,313,845..8,313,845 [+]

Nucleotide change:

G8313845A

Amino acid change:

G150S | CG7483-PA

Reported amino acid change:

G150S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Palacios et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | germline clone

(Palacios et al., 2004)

lethal

(Baker et al., 1991)

lethal | germline clone

(Palacios et al., 2004)

Phenotype Manifest In

cytoskeleton & oocyte | germ-line clone

(Palacios et al., 2004)

main body follicle cell | germline clone

(Palacios et al., 2004)

nucleus & oocyte | germ-line clone

(Palacios et al., 2004)

Detailed Description

Statement

Reference

Fully mutant germ-line clones cannot be made. However mosaic oocytes are seen. In these cysts, the mutant cells show an aberrant organisation of the actin cytoskeleton, nuclei of reduced size, and a detachment of the follicle cells (which is indicative of cell death).

(Palacios et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CG7483¹⁹/eIF4AIII[+] is a suppressor of visible phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2010)

Other

Statement

Reference

CG7483¹⁹/eIF4AIII[+], Df(2R)BSC600/Pym^SH1616 has viable phenotype

(Ghosh et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

CG7483¹⁹ is an enhancer of abdominal segment phenotype of btz¹, osk²

(Palacios et al., 2004)

CG7483¹⁹ is an enhancer of ovary phenotype of btz¹

(Palacios et al., 2004)

CG7483¹⁹ is an enhancer of primordial germ cell phenotype of btz¹

(Palacios et al., 2004)

Suppressor of

Statement

Reference

CG7483¹⁹/eIF4AIII[+] is a suppressor of eye phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

eIF4AIII¹⁹ dominantly suppresses the rough eye phenotype caused by Ras85D^V12.sev.

(Ashton-Beaucage et al., 2010)

The osk and stau localisation phenotypes seen in btz¹ oocytes are enhanced in combination with eIF4AIII¹⁹. The loss of pole cells and the associated loss of ovaries is also enhanced: penetrance of this phenotype increases from 6% to 31%, for loss of at least one ovary. Embryos produced by btz¹ females with one copy of osk² show a partial loss of abdominal segments. This phenotype is enhanced by the presence of eIF4AIII¹⁹.

(Palacios et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

2781