FlyBase Allele Report: Dmel\mei-9[A1]

General Information

Symbol

Dmel\mei-9^A1

Species

D. melanogaster

Name

FlyBase ID

FBal0012173

Feature type

allele

Associated gene

Dmel\mei-9

Associated Insertion(s)

Carried in Construct

Also Known As

mei-9^AT1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yamamoto and Wada, 1990, Smith, 1976)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C436T.

(Yildiz et al., 2004)

Amino acid replacement: R104term.

(Yildiz et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,317,487..4,317,487 [-]

Nucleotide change:

C4317487T

Reported nucleotide change:

C436T

Amino acid change:

R147term | mei-9-PA; R147term | mei-9-PB

Reported amino acid change:

R104term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. Identical to mei-9^A2 and mei-9^A3.

(Yildiz et al., 2004, Yamamoto and Wada, 1990, Smith, 1976)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | conditional

(Jassim et al., 2003)

larval brain | third instar larval stage

(Merigliano et al., 2017)

mitosis & nuclear chromosome

(Gatti, 1979)

nuclear chromosome | third instar larval stage

(Merigliano et al., 2017)

Detailed Description

Statement

Reference

mei-9^A1/mei-9^A1 third instar larval brains exhibit increased frequency of cells with chromosome aberrations but without telomeric fusions compared to controls.

(Merigliano et al., 2017)

Similarly to wild-type third-instar larvae, X-ray irradiation of mei-9^A1 mutants induces cell cycle arrest in wing imaginal disc cells.

(Kondo and Perrimon, 2011)

When homozygous mutants are UV irradiated a complete ablation of the eye is seen due to an increase in apoptotic cell death. Light-induced photorepair partially rescues the eye phenotype.

(Jassim et al., 2003)

Homozygotes are sensitive to methyl methanesulfonate and γ rays.

(Flores and Engels, 1999)

Homozygotes have an increased frequency of X chromosome non-disjunction.

(Yamamoto and Wada, 1990)

Approximately 20-28% of embryos hatch. Approximately 18% of mus201^D1 mei-9^A1 double mutant embryos hatch. Homozygous larvae derived from homozygous mothers are 35.1 times more sensitive to methyl methanesulfonate and 5.5 times more sensitive to UV light than wild-type. Homozygous mei-9^A1 or mei-9^A1 mus201^D1 embryonic cells in culture show no detectable unscheduled DNA synthesis (UDS) activity in response to methyl methanesulfonate, N-methyl-N-nitrosourea, X rays or UV light, over dose ranges in which wild-type cells show a strong dose-dependent UDS response.

(Dusenbery et al., 1983)

Larvae show wild-type sensitivity to formaldehyde.

(Alexandrov et al., 1982)

Homozygotes and hemizygotes show a higher frequency of spontaneous chromosome aberrations in neuroblast metaphases than wild-type larvae. The ratio of chromatid breaks to isochromatid breaks is 8.2-9.2. Approximately half of the breaks are heterochromatic and half are euchromatic. The breaks appear to be randomly distributed among the chromosomes. Breaks are 1.4-1.6 times more frequent in females than in males.

(Gatti, 1979)

Hemizygous male larvae are sensitive to methyl methanesulfonate (MMS). They show sensitivity to ultra violet (UV) light and X rays. 28.4% of eggs laid by homozygous females develop into adults that eclose.

(Smith, 1976)

mutagen sensitive reduces exchange mitotic instability

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

mei-9^A1, tws⁴³⁰ has lethal - all die before end of larval stage phenotype

(Merigliano et al., 2017)

mei-9^A1, mus81^Nhe has radiation sensitive phenotype

(Kondo and Perrimon, 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

mei-9^A1 has nuclear chromosome | third instar larval stage phenotype, enhanceable by tws⁴³⁰/tws⁴³⁰

(Merigliano et al., 2017)

mei-9^A1 has larval brain | third instar larval stage phenotype, enhanceable by tws⁴³⁰/tws⁴³⁰

(Merigliano et al., 2017)

Suppressor of

Statement

Reference

mei-9^A1/mei-9^A1 is a suppressor | partially of nuclear chromosome | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

mei-9^A1/mei-9^A1 is a suppressor | partially of larval brain | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

Other

Statement

Reference

mei-9^A1, mus81^Nhe has wing disc | third instar larval stage phenotype

(Kondo and Perrimon, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

In contrast to mei-9^A1 or mus81^Nhe single mutant larvae, X-ray irradiation of mei-9^A1, mus81^Nhe double mutant larvae induces cell cycle arrest in wing imaginal disc cells.

(Kondo and Perrimon, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6792

w¹ mei-9^A1/FM7h

Kyoto

101007

C(1)DX, y¹ f¹ / w¹ mei-9^A1

Notes on Origin

Discoverer

Alleles mei-9^A1, mei-9^A2 and mei-9^A3 contain the same molecular lesion.

(Yildiz et al., 2004)

Comments

The sex-linked recessive lethal test has been used to compare the induction of mutations by ethyl methanesulfonate and methyl methanesulfonate in spermatogenic stages of mei-9^A1 and wild-type males. Induced mutation rates in mei-9^A1 males are similar to wild-type for meiotic and post-meiotic stages, but for spermatogonial stages, mei-9^A1 males show a 4-8 fold increase in induced mutation rate compared to wild-type. The spontaneous mutation frequency in mei-9^A1 males is similar to wild-type.

(Smith et al., 1983)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

mei-9^A1

(Merigliano et al., 2017, Kondo and Perrimon, 2011)

mei-9^AT1

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Yamamoto and Wada, 1990, Yamamoto et al., 1990, Dusenbery et al., 1983, Smith et al., 1983, Lawlor, 1980, Gatti, 1979)

mei-9^ATI

(Alexandrov et al., 1982)

mei9^AT1

(Flores and Engels, 1999)

mus(1)110^A1

(Smith, 1976)

mus-110^AT1

mus110^A1

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close