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General Information
Symbol
Dmel\mei-9A1
Species
D. melanogaster
Name
FlyBase ID
FBal0012173
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
mei-9AT1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C4317487T

Reported nucleotide change:

C436T

Amino acid change:

R147term | mei-9-PA; R147term | mei-9-PB

Reported amino acid change:

R104term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. Identical to mei-9A2 and mei-9A3.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C436T.

Amino acid replacement: R104term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mei-9A1/mei-9A1 third instar larval brains exhibit increased frequency of cells with chromosome aberrations but without telomeric fusions compared to controls.

Similarly to wild-type third-instar larvae, X-ray irradiation of mei-9A1 mutants induces cell cycle arrest in wing imaginal disc cells.

When homozygous mutants are UV irradiated a complete ablation of the eye is seen due to an increase in apoptotic cell death. Light-induced photorepair partially rescues the eye phenotype.

Homozygotes are sensitive to methyl methanesulfonate and γ rays.

Homozygotes have an increased frequency of X chromosome non-disjunction.

Approximately 20-28% of embryos hatch. Approximately 18% of mus201D1 mei-9A1 double mutant embryos hatch. Homozygous larvae derived from homozygous mothers are 35.1 times more sensitive to methyl methanesulfonate and 5.5 times more sensitive to UV light than wild-type. Homozygous mei-9A1 or mei-9A1 mus201D1 embryonic cells in culture show no detectable unscheduled DNA synthesis (UDS) activity in response to methyl methanesulfonate, N-methyl-N-nitrosourea, X rays or UV light, over dose ranges in which wild-type cells show a strong dose-dependent UDS response.

Larvae show wild-type sensitivity to formaldehyde.

Homozygotes and hemizygotes show a higher frequency of spontaneous chromosome aberrations in neuroblast metaphases than wild-type larvae. The ratio of chromatid breaks to isochromatid breaks is 8.2-9.2. Approximately half of the breaks are heterochromatic and half are euchromatic. The breaks appear to be randomly distributed among the chromosomes. Breaks are 1.4-1.6 times more frequent in females than in males.

Hemizygous male larvae are sensitive to methyl methanesulfonate (MMS). They show sensitivity to ultra violet (UV) light and X rays. 28.4% of eggs laid by homozygous females develop into adults that eclose.

mutagen sensitive reduces exchange mitotic instability

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

In contrast to mei-9A1 or mus81Nhe single mutant larvae, X-ray irradiation of mei-9A1, mus81Nhe double mutant larvae induces cell cycle arrest in wing imaginal disc cells.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Alleles mei-9A1, mei-9A2 and mei-9A3 contain the same molecular lesion.

Comments
Comments

The sex-linked recessive lethal test has been used to compare the induction of mutations by ethyl methanesulfonate and methyl methanesulfonate in spermatogenic stages of mei-9A1 and wild-type males. Induced mutation rates in mei-9A1 males are similar to wild-type for meiotic and post-meiotic stages, but for spermatogonial stages, mei-9A1 males show a 4-8 fold increase in induced mutation rate compared to wild-type. The spontaneous mutation frequency in mei-9A1 males is similar to wild-type.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (15)