FlyBase Allele Report: Dmel\Zw10[16]

General Information

Symbol

Dmel\Zw10¹⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0012305

Feature type

allele

Associated gene

Dmel\Zw10

Associated Insertion(s)

Doc{}Zw10¹⁶

Carried in Construct

Also Known As

zw10^S1, l(1)zw10^S1

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Williams et al., 1992)

loss of function allele

(Williams and Goldberg, 1994)

Mutagen

spontaneous

(Williams et al., 1992, Schalet, 1986)

Progenitor genotype

Associated Insertion(s)

Doc{}Zw10¹⁶

Cytology

Description

Insertion of a 4.5-kb insertion of DNA at coordinate +1.7, judged to be a Doc element on the basis of restriction mapping.

(Williams et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant 3rd instar larval brains have a mitotic phenotype; 42.3% of anaphases are abnormal (showing lagging chromatids, chromatin bridges and nondisjunction) in untreated brains. Colchicine-treated mutant brains show 35.8% premature sister chromatid separation (compared to 0.96% in wild type) and have a mitotic index of 0.71 (compared to 2.44 in wild-type treated brains).

(Scaerou et al., 2001)

The duration of prometaphase in mutant spermatocytes is similar to that in wild-type spermatocytes. Prometaphase is not prolonged by treatment with taxol in mutant spermatocytes, in contrast to wild type. Chromosomes in mutant spermatocytes are less dynamic during early prometaphase than in wild-type spermatocytes, the rate of poleward chromosome motion is markedly reduced. The rate of anaphase poleward motion is reduced in mutant secondary spermatocytes.

(Savoian et al., 2000)

Transmission rate of Dp(1;f)J21A through females to progeny is 28%, mit(1)15 mutation has no effect on transmission.

(Cook et al., 1997)

Adult escaper males are sterile, testes are small and contain immotile sperm. Mutant onion stage spermatids vary considerably in size, indicative of chromosome missegregation during both meiotic divisions (nondisjunction during anaphase). Mistakes in chromosome behaviour are visualised by lagging chromosomes and chromatin bridges. Meiotic as well as mitotic defects are specific for events occurring either at anaphase onset or during anaphase proper. Mutation does not cause precocious sister chromatid separation (PSCS) during the first meiotic division but does affect cytokinesis (spermatids containing more than one nucleus per nebenkern).

(Williams et al., 1996)

Embryos derived from homozygous germline clones generally terminate development in the late syncytial blastoderm stages, although 15-25% of develop to later stages of embryogenesis, and 1% hatch into larvae. Mitotic synchrony is lost in embryos derived from homozygous germline clones. Chromatin bridges between nuclei, unequal segregation of chromosomes and lagging chromatids are seen at anaphase.

(Williams and Goldberg, 1994)

Brain cells are hyperploid. Aneuploidy involves improper chromosome segregation at anaphase: precocious sister chromatid separation. Mitotic index and the ratio of numbers of cells in anaphase to the total number of mitotic figures in larval brains is similar in wild type.

(Williams et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Zw10¹⁶ has abnormal mitotic cell cycle phenotype, non-enhanceable by rod^X-5

(Scaerou et al., 2001)

NOT suppressed by

Statement

Reference

Zw10¹⁶ has abnormal mitotic cell cycle phenotype, non-suppressible by rod^X-5

(Scaerou et al., 2001)

NOT Enhancer of

Statement

Reference

Zw10¹⁶ is a non-enhancer of abnormal mitotic cell cycle phenotype of rod^X-5

(Scaerou et al., 2001)

NOT Suppressor of

Statement

Reference

Zw10¹⁶ is a non-suppressor of abnormal mitotic cell cycle phenotype of rod^X-5

(Scaerou et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Zw10¹⁶ has nuclear chromosome phenotype, non-enhanceable by rod^X-5

(Scaerou et al., 2001)

Zw10¹⁶ has mitotic anaphase phenotype, non-enhanceable by rod^X-5

(Scaerou et al., 2001)

NOT suppressed by

Statement

Reference

Zw10¹⁶ has nuclear chromosome phenotype, non-suppressible by rod^X-5

(Scaerou et al., 2001)

Zw10¹⁶ has mitotic anaphase phenotype, non-suppressible by rod^X-5

(Scaerou et al., 2001)

NOT Enhancer of

Statement

Reference

Zw10¹⁶ is a non-enhancer of nuclear chromosome phenotype of rod^X-5

(Scaerou et al., 2001)

Zw10¹⁶ is a non-enhancer of mitotic anaphase phenotype of rod^X-5

(Scaerou et al., 2001)

NOT Suppressor of

Statement

Reference

Zw10¹⁶ is a non-suppressor of nuclear chromosome phenotype of rod^X-5

(Scaerou et al., 2001)

Zw10¹⁶ is a non-suppressor of mitotic anaphase phenotype of rod^X-5

(Scaerou et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Abnormal anaphases and premature sister chromatid separation occur at the same frequency in mit(1)15¹⁶ rod^X-5 double mutants as in either single mutant alone. There is no additive effect; the range of defects is quantitatively and qualitatively comparable with that for either single mutant.

(Scaerou et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Zw10¹⁶ is rescued by Zw10^+t4.6

(Williams et al., 1992)

Comments

Lethal phenotype can be rescued by P element mediated transformation of a wild type mit(1)15 gene copy.

(Williams et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Schalet.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Zw10¹⁶

l(1)6-99

(Schalet, 1986)

l(1)zw10^S1

(Cook et al., 1997, Williams et al., 1992)

mit(1)15¹⁶

zw10^S1

(Wainman et al., 2009, Williams et al., 2003, Scaerou et al., 2001, Savoian et al., 2000, Basu et al., 1999, Starr et al., 1998, Williams et al., 1996, Williams and Goldberg, 1994)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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