FlyBase Allele Report: Dmel\opa[8]

General Information

Symbol

Dmel\opa⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0013272

Feature type

allele

Associated gene

Dmel\opa

Associated Insertion(s)

Carried in Construct

Also Known As

opa^IIP32, opa^2P32

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Soluri et al., 2020)

loss of function allele

(Lee et al., 2007)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: C298Y.

(Soluri et al., 2020)

Mutation is within a critical Cys residue within the first predicted C2H2 Zn-finger.

(Soluri et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:4,853,998..4,853,998 [+]

Nucleotide change:

G4853998A

Amino acid change:

C298Y | opa-PA; C298Y | opa-PB

Reported amino acid change:

C298Y

Comment:

Mutation in a critical Cys residue within the first predicted C2H2 Zn-finger.

(Soluri et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage (with opa^12.3)

(Lee et al., 2007)

lethal - all die during embryonic stage (with opa^32.3)

(Lee et al., 2007)

lethal - all die during embryonic stage (with opa^M)

(Lee et al., 2007)

lethal - all die during embryonic stage (with opa^Q)

(Lee et al., 2007)

some die during embryonic stage (with opa^12.3)

(Lee et al., 2007)

some die during embryonic stage (with opa^32.3)

(Lee et al., 2007)

some die during embryonic stage (with opa^M)

(Lee et al., 2007)

some die during embryonic stage (with opa^Q)

(Lee et al., 2007)

Phenotype Manifest In

embryo

(Soluri et al., 2020)

ventral denticle belt

(Baumgartner et al., 1994)

Detailed Description

Statement

Reference

Severe cuticle phenotype with partial fusion, nearly complete and complete fusion of adjacent denticle belts.

(Baumgartner et al., 1994)

strong allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

dpp^TgR46.1/dpp[+], opa⁸ has visible phenotype

(Lee et al., 2007)

Phenotype Manifest In

Other

Statement

Reference

dpp^TgR46.1/dpp[+], opa⁸ has eye phenotype

(Lee et al., 2007)

dpp^TgR46.1/dpp[+], opa⁸ has vibrissal bristle phenotype

(Lee et al., 2007)

dpp^TgR46.1/dpp[+], opa⁸ has maxillary palp phenotype

(Lee et al., 2007)

dpp^TgR46.1/dpp[+], opa⁸ has adult head | ventral phenotype

(Lee et al., 2007)

dpp^TgR46.1/dpp[+], opa⁸ has gena phenotype

(Lee et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

In dpp^TgR46.1/+; opa⁸/+ flies the ventral head is disrupted, the eye is round rather than oval, the sensory vibrissae on the ventral side of the eye are bunched, the maxillary palps are missing, reduced or duplicated, and gena tissue appears to be missing.

(Lee et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Lee et al., 2007)

(Lee et al., 2007)

(Lee et al., 2007)

(Lee et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5335

P{ftz-lacC}1, opa⁸/TM3, Sb¹

Kyoto

101742

ru¹ klar¹ opa⁸ / TM3, y⁺ ru¹ klar¹ kni^ri-1 p^p vvl^sep Sb¹ Ubx^bx-34e e^s Ser¹

Notes on Origin

Discoverer

Comments

prd RNA expression has been studied in opa⁸ embryos.

(Baumgartner and Noll, 1990)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

opa^2P32

(Lee et al., 2007, Mohler, 1995)

opa⁸

(Mendoza-García et al., 2017, Lee et al., 2007)

opa^II32

(DiNardo and O'Farrell, 1987)

opa^IIP32

(Soluri et al., 2020, Azpiazu et al., 1996, Harbecke and Lengyel, 1995, Baumgartner et al., 1994, Irvine and Wieschaus, 1994, Li et al., 1993, Baumgartner and Noll, 1990)

opa^IIP

(Moore et al., 1998, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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