FlyBase Allele Report: Dmel\pcx[3]

General Information

Symbol

Dmel\pcx³

Species

D. melanogaster

Name

FlyBase ID

FBal0013629

Feature type

allele

Associated gene

Dmel\pcx

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Yamakawa et al., 2012)

Mutagen

ethyl methanesulfonate

(Mohler, 1977)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Yamakawa et al., 2012)

Amino acid replacement: W2030term.

(Yamakawa et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,232,264..2,232,264 [+]

Nucleotide change:

G2232264A

Reported nucleotide change:

G?A

Amino acid change:

W2030term | pcx-PC; W2034term | pcx-PE; W2030term | pcx-PF

Reported amino acid change:

W2030term

Comment:

TGG to TGA change in the W2030 codon.

(Yamakawa et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg | maternal effect

(Mohler, 1977)

endoplasmic reticulum | maternal effect

(Yamakawa et al., 2012)

Detailed Description

Statement

Reference

Mutant embryos lacking both maternal and zygotic pcx function show a neurogenic phenotype. This phenotype can be paternally rescued in 80% of embryos.

The endoplasmic reticulum (ER) appears to be normal in mutant embryos lacking both maternal and zygotic pcx at stage 5. However, ER defects are apparent at stage 9 in these embryos, and enlarged ER is seen at stage 14, predominantly in the region corresponding to the dorsal epidermis of wild-type embryos at this stage.

(Yamakawa et al., 2012)

Embryonic defects detectable during blastoderm stage.

(Mohler and Carroll, 1984)

Homozygous females lay eggs that fail to hatch.

(Mohler, 1977)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pcx³ has neurogenic phenotype | maternal effect | embryonic stage phenotype, suppressible | partially by N^ΔECN.hs

(Yamakawa et al., 2012)

pcx³ has neurogenic phenotype | maternal effect | embryonic stage phenotype, suppressible | partially by N^ICN.UAS/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

pcx³ has neurogenic phenotype | maternal effect | embryonic stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Xbp1^UAS.RB

(Yamakawa et al., 2012)

pcx³ has neurogenic phenotype | maternal effect | embryonic stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Hsc70-3^UAS.cEa

(Yamakawa et al., 2012)

pcx³ has neurogenic phenotype | maternal effect | embryonic stage phenotype, suppressible | partially by Hsc70-3^K97S.UAS/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

pcx³ has neurogenic phenotype | maternal effect | embryonic stage phenotype, suppressible | partially by Ofut1^UAS.cSa/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

pcx³ has endoplasmic reticulum | maternal effect phenotype, suppressible by N^ICN.UAS/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

pcx³ has endoplasmic reticulum | maternal effect phenotype, suppressible by Scer\GAL4^arm.PS/Xbp1^UAS.RB

(Yamakawa et al., 2012)

pcx³ has endoplasmic reticulum | maternal effect phenotype, suppressible by Hsc70-3^K97S.UAS/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

pcx³ has endoplasmic reticulum | maternal effect phenotype, suppressible by Ofut1^UAS.cSa/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of N^ΔECN.hs in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype is 90% of embryos, while the phenotype is not rescued in the remaining 10% of embryos.

Expression of N^ICN.Scer\UAS under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 44% of embryos are rescued, 48% are partially rescued and 8% are not rescued. The endoplasmic reticulum defects are also rescued.

Expression of Xbp1^Scer\UAS.RB under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 31% of embryos are fully rescued, 43% are partially rescued and 26% are not rescued. The endoplasmic reticulum defects are also rescued in 74% of cases.

Expression of Hsc70-3^Scer\UAS.cEa under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 67% of embryos are partially rescued and 33% are not rescued.

Expression of Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 29% of embryos are fully rescued, 38% are partially rescued and 33% are not rescued. The endoplasmic reticulum defects are also rescued in 67% of cases.

Expression of O-fut1^Scer\UAS.cSa under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function rescues the neurogenic phenotype and endoplasmic reticulum defects in 31% of cases.

(Yamakawa et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

pcx³ is partially rescued by pcx^UAS.EGFP/Scer\GAL4^arm.PS

(Yamakawa et al., 2012)

Comments

Expression of pcx^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^arm.PS rescues the neurogenic phenotype seen in pcx³ embryos which lack maternal and zygotic pcx function (73% of embryos are rescued). The endoplasmic reticulum defects are also rescued.

In pcx³ embryos which lack maternal and zygotic pcx function, clones of cells expressing pcx^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PT do not assume a neural fate, while the surrounding cells become neurons.

(Yamakawa et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

4592

y¹ pcx³ cv¹ v¹ f¹/FM6

Notes on Origin

Discoverer

Mohler.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

12-1012

fs(1)M10^12-1012

(Mohler and Carroll, 1984)

pcx³

(Das et al., 2020, Yamakawa et al., 2018, Yamakawa et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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