FlyBase Allele Report: Dmel\Xbp1[UAS.RB]

General Information

Symbol

Dmel\Xbp1^UAS.RB

Species

D. melanogaster

Name

FlyBase ID

FBal0230352

Feature type

allele

Associated gene

Dmel\Xbp1

Associated Insertion(s)

Carried in Construct

P{UAS-Xbp1.RB}

Also Known As

UAS-Xbp1-RB, UAS-Xbp1s, UAS::xbp1^spliced, xbp1-RB

Key Links

Transgenic product class

wild_type

(Ryoo et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ryoo et al., 2007)

Mutagen

in vitro construct

(Ryoo et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Xbp1.RB}

Cytology

Description

UASt regulatory sequences drive expression of the Xbp1-RB isoform.

(Ryoo et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Xbp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Scer\GAL4^Act5C.PP

(Arsham and Neufeld, 2009)

eye, with Scer\GAL4^GMR.PF

(Ryoo et al., 2007)

eye photoreceptor cell, with Scer\GAL4^ninaE.PT

(Ryoo et al., 2007)

lysosome & larval fat body | somatic clone | cell autonomous, with Scer\GAL4^Act5C.PP

(Arsham and Neufeld, 2009)

midgut | adult stage | somatic clone, with Scer\GAL4^unspecified

(Wang et al., 2014)

secondary tracheal branch, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

tracheal section, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

Detailed Description

Statement

Reference

Expression of Xbp1^UAS.RB under the control of Scer\GAL4^bs.Term causes air-filling defects and reduced branch numbers in the trachea terminal cells region.

(Ríos-Barrera et al., 2017)

Overexpression of Xbp1^Scer\UAS.RB in class IV da neurons (Scer\GAL4^ppk.PU) or class III da neurons (Scer\GAL4^19-12 in combination with Scer\GAL80^repo.PL) significantly enhances axon regeneration.

(Song et al., 2015)

Expression of Xbp1^Scer\UAS.RB under the control of Scer\GAL4^bun-GSG5961, using RU486 food supplementation to induce the expression, extends adult lifespan relative to controls.

(Wang et al., 2015)

The temporally regulated expression (by Gal80[ts]) of Xbp1^UAS.RB, for 5 days under the control of Scer\GAL4^NP5130, does not lead to a significant change in the proportion of mitotic cells in the adult midgut, as compared to controls; clonal expression under the control of Scer\GAL4^unspecified in adult midgut clones leads to a significant decrease in clone cell number at 7 days after clone induction, as compared to control clones.

(Wang et al., 2014)

Xbp1^Scer\UAS.RB clones in fat bodies from well-fed early third instar larvae (generated using Scer\GAL4^Act5C.PP) accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.

(Arsham and Neufeld, 2009)

Expression of Xbp1^Scer\UAS.RB under the control of Scer\GAL4^GMR.PF generates small, rough eyes with enhanced cell death.

(Ryoo et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^unspecified, Xbp1^UAS.RB has decreased cell number | somatic clone | adult stage phenotype, non-suppressible | somatic clone by cnc^VL110/cnc^VL110

(Wang et al., 2014)

Scer\GAL4^GMR.PF, Xbp1^UAS.RB has increased cell death phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Ryoo et al., 2007)

Suppressor of

Statement

Reference

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Ero1L^GD3577, Scer\GAL4^NP5130

(Wang et al., 2015)

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor of increased cell number | adult stage phenotype of N^RNAi.P.UAS, Scer\GAL4^NP5130

(Wang et al., 2014)

Scer\GAL4^arm.PS/Xbp1^UAS.RB is a suppressor | partially of neurogenic phenotype | maternal effect | embryonic stage phenotype of pcx³

(Yamakawa et al., 2012)

Other

Statement

Reference

Scer\GAL4^unspecified, Xbp1^UAS.RB, cnc^VL110 has decreased cell number | somatic clone | adult stage phenotype

(Wang et al., 2014)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^unspecified, Xbp1^UAS.RB has midgut | somatic clone | adult stage phenotype, non-suppressible | somatic clone by cnc^VL110/cnc^VL110

(Wang et al., 2014)

Scer\GAL4^GMR.PF, Xbp1^UAS.RB has eye phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Ryoo et al., 2007)

Suppressor of

Statement

Reference

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | adult stage phenotype of Ero1L^GD3577, Scer\GAL4^NP5130

(Wang et al., 2015)

Xbp1^UAS.RB/Scer\GAL4^NP5130 is a suppressor of midgut | adult stage phenotype of Scer\GAL4^NP5130, hep^UAS.cBa

(Wang et al., 2014)

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | adult stage phenotype of N^RNAi.P.UAS, Scer\GAL4^NP5130

(Wang et al., 2014)

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor of enteroendocrine cell | adult stage phenotype of N^RNAi.P.UAS, Scer\GAL4^NP5130

(Wang et al., 2014)

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor of midgut | adult stage phenotype of N^RNAi.P.UAS, Scer\GAL4^NP5130

(Wang et al., 2014)

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a suppressor | partially of midgut | adult stage phenotype of InR^UAS.cUa, Scer\GAL4^NP5130

(Wang et al., 2014)

Scer\GAL4^arm.PS/Xbp1^UAS.RB is a suppressor of endoplasmic reticulum | maternal effect phenotype of pcx³

(Yamakawa et al., 2012)

NOT Suppressor of

Statement

Reference

Xbp1^UAS.RB, Scer\GAL4^NP5130 is a non-suppressor of midgut | adult stage phenotype of Prx2^RNAi.UAS.cUa, Scer\GAL4^NP5130

(Wang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The increased cell proliferation in the adult gut observed in flies expressing Ero1L^GD3577 under the control of Scer\GAL4^esg-NP5130 (and using tub-Gal80[ts] to limit the time of expression) is suppressed by co-expression of Xbp1^Scer\UAS.RB.

(Wang et al., 2015)

Expression of Xbp1^Scer\UAS.RB under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 31% of embryos are fully rescued, 43% are partially rescued and 26% are not rescued. The endoplasmic reticulum defects are also rescued in 74% of cases.

(Yamakawa et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa does not suppress the small, rough eyes with enhanced cell death phenotype of Xbp1^Scer\UAS.RB, Scer\GAL4^GMR.PF flies.

(Ryoo et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Xbp1^Scer\UAS.RB

Xbp1^UAS.RB

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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