FlyBase Allele Report: Dmel\pnr[1]

General Information

Symbol

Dmel\pnr¹

Species

D. melanogaster

Name

FlyBase ID

FBal0013908

Feature type

allele

Associated gene

Dmel\pnr

Associated Insertion(s)

Carried in Construct

Also Known As

pnr^7G

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Gajewski et al., 1999)

Mutagen

ethyl methanesulfonate

(Heitzler et al., 1996, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: W180term.

(Alvarez et al., 2003)

Nucleotide substitution: G1034A.

(Alvarez et al., 2003)

Molecular lesion unknown.

(Heitzler et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:16,039,098..16,039,098 [+]

Nucleotide change:

G16039098A

Reported nucleotide change:

G1034A

Amino acid change:

W180term | pnr-PA; W128term | pnr-PB; W180term | pnr-PC; W128term | pnr-PD

Reported amino acid change:

W180term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Alvarez et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Gajewski et al., 1999, Heitzler et al., 1996)

some die during embryonic stage | recessive

(Gajewski et al., 1999, Heitzler et al., 1996)

Phenotype Manifest In

adult heart

(Qian and Bodmer, 2009)

dorsal vessel wall cell

(Sellin et al., 2006, Alvarez et al., 2003, Gajewski et al., 1999)

embryonic dorsal epidermis

(Heitzler et al., 1996)

embryonic epidermis

(Ricos et al., 1999)

embryonic pericardial cell

(Alvarez et al., 2003)

larval pericardial cell | ectopic

(Gajewski et al., 1999)

mesothoracic cleft

(Heitzler et al., 1996)

Detailed Description

Statement

Reference

Heterozygous adults show an increase in heart arrest/fibrillation compared to control flies upon electrical pacing of the heart. The severity of the defect increases with age.

Heterozygous adults show an increase in irregular heart rate compared to controls. The mean diastolic interval length is increased compared to controls.

(Qian and Bodmer, 2009)

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for pnr¹ do not show defects in the establishment or maintenance of dendritic tiling.

(Parrish et al., 2007)

In pnr¹ mutant embryos, the number of cardioblasts is strongly reduced and the embryos fail to form a closed heart tube.

(Sellin et al., 2006)

In mutant embryos most cardioblasts so not develop, although short stretches of cardioblast still arise. A strong decrease in pericardial cells is also seen.

(Alvarez et al., 2003)

Homozygous embryos have a severe dorsal hole. Cardial cells are absent, and there is an excess of eve-expressing pericardial cells.

(Gajewski et al., 1999)

Homozygous embryos show bunching of epidermal segments at the end of dorsal closure.

(Ricos et al., 1999)

Homozygotes exhibit a dorsal hole that is broader than that of pnr^VX6 or Df(3R)sbd45 homozygotes. Heterozygotes with pnr^D3 give adult escapers and heterozygotes with pnr^VX5 show extra dorsocentrals. Mitotic clones in the thorax adjacent to the thoracic midline cause a sizeable bilateral cleft involving most of the scutum causing the entire scutellum to disappear.

(Heitzler et al., 1996)

embryonic lethal. Dorsal anterior of embryo open.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

pnr¹ has scutellum phenotype, enhanceable by XNP^UY3132/Scer\GAL4^455.2

(Valadez-Graham et al., 2012)

NOT Enhanced by

Statement

Reference

pnr¹ has adult heart phenotype, non-enhanceable by tin³⁴⁶/tin[+]

(Qian and Bodmer, 2009)

pnr¹ has adult heart phenotype, non-enhanceable by Df(3R)GC14/+

(Qian and Bodmer, 2009)

pnr¹ has adult heart phenotype, non-enhanceable by H15^nmr-614/H15[+]

(Qian and Bodmer, 2009)

pnr¹ has adult heart phenotype, non-enhanceable by Df(2L)Exel6012/+

(Qian and Bodmer, 2009)

pnr¹ has dorsal vessel wall cell phenotype, non-enhanceable by pnt^S012309

(Alvarez et al., 2003)

NOT suppressed by

Statement

Reference

pnr¹ has adult heart phenotype, non-suppressible by tin³⁴⁶/tin[+]

(Qian and Bodmer, 2009)

pnr¹ has adult heart phenotype, non-suppressible by Df(3R)GC14/+

(Qian and Bodmer, 2009)

pnr¹ has adult heart phenotype, non-suppressible by H15^nmr-614/H15[+]

(Qian and Bodmer, 2009)

pnr¹ has adult heart phenotype, non-suppressible by Df(2L)Exel6012/+

(Qian and Bodmer, 2009)

pnr¹ has dorsal vessel wall cell phenotype, non-suppressible by pnt^S012309

(Alvarez et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of XNP^UY3132 under the control of Scer\GAL4^455.2 enhances the penetrance of the scutellar phenotypes seen in pnr¹ heterozygotes.

(Valadez-Graham et al., 2012)

The increase in heart arrest/fibrillation upon electrical pacing that is seen in pnr¹/+ adults is not significantly altered by tin³⁴⁶/+ or Df(3R)GC14/+.

The increase in heart arrest/fibrillation upon electrical pacing that is seen in pnr¹/+ adults is not significantly altered by H15^nmr-614/+ or Df(2L)Exel6012/+.

(Qian and Bodmer, 2009)

In pnr¹, pnt^S012309 embryos local overproduction of cardioblasts are seen as seen in pnr¹ embryos.

(Alvarez et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

pnr¹ is partially rescued by pnr^UAS.cHa/Scer\GAL4^Mef2.PR

(Qian and Bodmer, 2009)

pnr¹ is partially rescued by pnr^UAS.cHa/Scer\GAL4^tin.CΔ4

(Qian and Bodmer, 2009)

pnr¹ is partially rescued by pnr^UAS.cHa/Scer\GAL4^twi.PG/Scer\GAL4^how-24B

(Qian and Bodmer, 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3106

ru¹ hry¹ Diap1^th-1 st¹ cu¹ pnr¹ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

Kyoto

106847

ru¹ hry¹ Diap1^th-1 st¹ cu¹ pnr¹ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Class 2 pnr allele: lethal recessive allele. Allelic series according to the extent of the dorsal hole: pnr¹ > Df(3R)sbd45 = pnr^VX6 = pnr^VP8 = pnr^D1 > pnr^D3.

(Heitzler et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

pannier^7G

(Arora et al., 1995)

pnr¹

(Neidviecky and Deng, 2023, Valadez-Graham et al., 2012, Qian and Bodmer, 2009, Parrish et al., 2007, Sellin et al., 2006)

pnr^7G111

(Heitzler et al., 1996)

pnr^7G

(Ricos et al., 1999, Nelson and Szauter, 1992, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

Open Close