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General Information
Symbol
Dmel\pnr1
Species
D. melanogaster
Name
FlyBase ID
FBal0013908
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
pnr7G
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G16039098A

Reported nucleotide change:

G1034A

Amino acid change:

W180term | pnr-PA; W128term | pnr-PB; W180term | pnr-PC; W128term | pnr-PD

Reported amino acid change:

W180term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W180term.

Nucleotide substitution: G1034A.

Molecular lesion unknown.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous adults show an increase in heart arrest/fibrillation compared to control flies upon electrical pacing of the heart. The severity of the defect increases with age.

Heterozygous adults show an increase in irregular heart rate compared to controls. The mean diastolic interval length is increased compared to controls.

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for pnr1 do not show defects in the establishment or maintenance of dendritic tiling.

In pnr1 mutant embryos, the number of cardioblasts is strongly reduced and the embryos fail to form a closed heart tube.

In mutant embryos most cardioblasts so not develop, although short stretches of cardioblast still arise. A strong decrease in pericardial cells is also seen.

Homozygous embryos have a severe dorsal hole. Cardial cells are absent, and there is an excess of eve-expressing pericardial cells.

Homozygous embryos show bunching of epidermal segments at the end of dorsal closure.

Homozygotes exhibit a dorsal hole that is broader than that of pnrVX6 or Df(3R)sbd45 homozygotes. Heterozygotes with pnrD3 give adult escapers and heterozygotes with pnrVX5 show extra dorsocentrals. Mitotic clones in the thorax adjacent to the thoracic midline cause a sizeable bilateral cleft involving most of the scutum causing the entire scutellum to disappear.

embryonic lethal. Dorsal anterior of embryo open.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

pnr1 has adult heart phenotype, non-enhanceable by tin346/tin[+]

pnr1 has adult heart phenotype, non-enhanceable by Df(3R)GC14/+

pnr1 has adult heart phenotype, non-enhanceable by H15nmr-614/H15[+]

pnr1 has adult heart phenotype, non-enhanceable by Df(2L)Exel6012/+

pnr1 has cardioblast phenotype, non-enhanceable by pntS012309

NOT suppressed by
Statement
Reference

pnr1 has adult heart phenotype, non-suppressible by tin346/tin[+]

pnr1 has adult heart phenotype, non-suppressible by Df(3R)GC14/+

pnr1 has adult heart phenotype, non-suppressible by H15nmr-614/H15[+]

pnr1 has adult heart phenotype, non-suppressible by Df(2L)Exel6012/+

pnr1 has cardioblast phenotype, non-suppressible by pntS012309

Additional Comments
Genetic Interactions
Statement
Reference

Expression of XNPUY3132 under the control of Scer\GAL4455.2 enhances the penetrance of the scutellar phenotypes seen in pnr1 heterozygotes.

The increase in heart arrest/fibrillation upon electrical pacing that is seen in pnr1/+ adults is not significantly altered by tin346/+ or Df(3R)GC14/+.

The increase in heart arrest/fibrillation upon electrical pacing that is seen in pnr1/+ adults is not significantly altered by H15nmr-614/+ or Df(2L)Exel6012/+.

In pnr1, pntS012309 embryos local overproduction of cardioblasts are seen as seen in pnr1 embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Class 2 pnr allele: lethal recessive allele. Allelic series according to the extent of the dorsal hole: pnr1 > Df(3R)sbd45 = pnrVX6 = pnrVP8 = pnrD1 > pnrD3.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (13)