FlyBase Allele Report: Dmel\Polr2A[4]

General Information

Symbol

Dmel\Polr2A⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0014661

Feature type

allele

Associated gene

Dmel\Polr2A

Associated Insertion(s)

Carried in Construct

Also Known As

RpII215^C4, C4, RpII215⁴

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Brickey and Greenleaf, 1995, Chen et al., 1993, Jokerst et al., 1989)

Progenitor genotype

Cytology

Description

Protein carries all of the CTD repeats.

(Brickey and Greenleaf, 1995)

Amino acid replacement: R741H. Nucleotide substitution: G3973A.

(Chen et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,567,146..11,567,146 [-]

Nucleotide change:

G11567146A

Reported nucleotide change:

G3973A

Amino acid change:

R741H | Polr2A-PA

Reported amino acid change:

R741H

(Chen et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant

(Jokerst et al., 1989, Mortin and Kaufman, 1982)

viable

(Krasnoselskaya et al., 1998, Chen et al., 1993, Voelker et al., 1985)

visible | dominant

(Mortin et al., 1988)

Phenotype Manifest In

capitellum

(Chen et al., 1993, Mortin et al., 1988)

Detailed Description

Statement

Reference

Heterozygotes raised on α-amanitin containing food show the Ubx effect mutant phenotype and are indistinguishable from flies raised on normal food.

(Burke et al., 1996)

Exhibits the Ubx effect: heterozygotes partially transform the capitellum of the haltere into the wing blade.

(Chen et al., 1993)

Amanitin resistant mutation.

(Jokerst et al., 1989)

The capitellum of the haltere shows a partial transformation to wing blade in heterozygotes. This transformation is suppressed in trans with RpII215^H1 and RpII215^K2, and RpII215^K1 at 29^oC. Enhances the bristle duplication phenotype of Dl⁷ heterozygotes.

(Mortin et al., 1988)

α-amanitin-resistant mutation.

(Mortin and Kaufman, 1982)

This allele was selected as an amanitin resistant mutation; RpII215⁴/+ females produce equal amounts or amanitin-sensitive and -resistant enzyme and are themselves amanitin resistant. Generally a viable allele, but survival in combination with RpII215⁶³ and RpII215⁸⁶ reduced and with RpII215⁵¹ and RpII215⁷⁸ survival is zero. RNA polymerase isolated from embryos homozygous for this allele is 250 times less sensitive to inhibition by α amanitin than that from wild type; resistance attributable to reduced amanitin binding; otherwise RNA polymerase normal in all respects except that there is some reduction in activity in the presence of Mg²⁺; stable to thermal denaturation (Coulter and Greenleaf, 1982). Flies carrying both RpII215⁴ and RpII215⁺ display an Ultrabithorax-like phenotype, with enlarged halteres and an enhancing effect on expression of Ubx (Greenleaf, Weeks, Voelker, Ohnishi and Dickson, 1980; Voelker, Wisely, Huang and Gyurkovics, 1985). Measurements of haltere size in TM6/+ females of various constitutions produced the following: 4/+/+ > 4/+ > 4/4/+ > 4/4 = 4/0 = +/+. The haltere effect of RpII215⁴ is abolished in heteroallelic combination with RpII215⁷, RpII215^H1 and RpII215^K2. RpII215⁴ decreases the Ubx effect of RpII215^Ubl. Heterozygotes also exhibit increased numbers of duplicated bristles in Dl/+ (Mortin et al.). Males carrying RpII215⁴ on their X plus a normal allele on Dp(1;Y)y⁺v⁺#3 are nearly sterile and those carrying the normal allele instead on Dp(1;2)v⁺65b are sterile (Voelker et al.). viable

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Polr2A⁴ is an enhancer of visible phenotype of Iswi^{K159R.UAS.Tag:HA}, Scer\GAL4^ey.PH

(Armstrong et al., 2005)

Polr2A⁴ is an enhancer of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

Other

Statement

Reference

Marcal1^del, RpII215[+]/Polr2A⁴ has partially lethal - majority die | embryonic stage phenotype

(Baradaran-Heravi et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

Polr2A⁴ is an enhancer of eye phenotype of Iswi^{K159R.UAS.Tag:HA}, Scer\GAL4^ey.PH

(Armstrong et al., 2005)

Polr2A⁴ is an enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

Other

Statement

Reference

Marcal1^del, RpII215[+]/Polr2A⁴ has egg phenotype

(Baradaran-Heravi et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Marcal1^del/Marcal1^del RpII215⁴/+ embryos show a reduced hatching rate compared to controls at 20[o]C.

The eggs laid by Marcal1^del/Marcal1^del RpII215⁴/+ are smaller than normal.

(Baradaran-Heravi et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3663

v¹ Polr2A⁴

Notes on Origin

Discoverer

Comments

Degree of transformation of the haltere when heterozygous: RpII215^H1 < RpII215⁷ < RpII215^K2 < RpII215⁴ < RpII215^Ubl.

(Mortin et al., 1988)

RNA polymerase II purified from RpII215⁴ embryos has a lower elongation rate than normal and is less efficient at reading through intrinsic elongation blocks. The response of the mutant enzyme to TfIIS and NH₄⁺ ions is similar to the response of the wild-type enzyme. RNA polymerase II purified from RpII215⁴ embryos is slower than wild-type RNA polymerase II or RNA polymerase II purified from RpII140^S1 embryos at generating the final pattern of shortened transcripts during extended TfIIS mediated pyrophosphorolysis. These results suggest that the RpII215⁴ mutation affects the movement of RNA polymerase II.

(Chen et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

(de la Mata et al., 2003, Chen et al., 1996, Chen et al., 1993, Mortin et al., 1988, Wilson and Fabian, 1986)

Polr2A⁴

RpII215⁴

(Mao et al., 2016, Baradaran-Heravi et al., 2012)

RpII215^C4

(Liu et al., 2017, Rieder et al., 2013, Pinto et al., 2011, Brickey and Greenleaf, 1995, Jokerst et al., 1989, Voelker et al., 1989, Mortin et al., 1988, Voelker et al., 1985)

RpII^C4

(Mortin and Kaufman, 1982)

Ubl^C4

Name Synonyms

Secondary FlyBase IDs

References (21)

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