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General Information
Symbol
Dmel\sa1
Species
D. melanogaster
Name
FlyBase ID
FBal0015120
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotides 145-558 are deleted and replaced with a 158bp insertion. This leads to a frameshift after codon 47.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

sa1/sa2 adult heterozygote males show morphological defects of the nuclear lamina in stage S4-S6 spermatocytes (the lamina has distinctly more ballon-like shape, lacking the deformations that correlate with chromosome distribution seen in wild-type), while in stage S3 the lamina is of wild-type appearance.

sa1/sa2 mutant spermatocytes exhibit meiotic arrest and other defects, including bivalent chromosomes condensing to spherical shapes away from the nuclear periphery, and failure of the nucleolus to fully break down.

sa1/sa2 males are sterile due to arrested spermatogenesis at spermatocyte stage.

Early stages of spermatogenesis up through mature primary spermatocytes are present and normal. Postmeiotic stages are completely absent. Mutant testes have an abundance of mature primary spermatocytes with large, round nuclei and prominent nucleoli. Triple mutant aly1, can1, sa1 homozygotes are female fertile and male sterile, with similar effects on testis squashes as sa1 and can1. Spermatocytes arrest in the G2/M transition of meiosis I with partial chromosome condensation.

male-sterile Primary spermatocytes arrested premeiotically but apparently after attaining full size; testes become filled with mature primary spermatocytes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

sa+t3.2 fully rescues the spermatocyte arrest and male sterility phenotypes of sa1/sa2 males.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (22)