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General Information
Symbol
Dmel\Tb1
Species
D. melanogaster
Name
FlyBase ID
FBal0016730
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

An in-frame 15 bp deletion in Tb. One of three mapped lesions in Tb in mutant.

Comment:

69bp in-frame deletion of 23 amino acid residues. One of three mapped lesions in Tb in Tb1 mutant strain.

Nucleotide change:

T26657334G

Reported nucleotide change:

T/G

Amino acid change:

S249A | Tb-PA

Reported amino acid change:

S?A

Comment:

Missense mutation in Tb. One of three mapped lesions in Tb in mutant.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

In-frame 15bp deletion in the second exon (coordinates 3R:26656728..26656742 , release 6 genome), 69bp in-frame deletion of 23 amino acid residues ( 3R:26657089..26657157 ) and a T to G (Ser to Ala) mutation at 3R:26657334 .

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Tb1 homozygous larvae start to exhibit abnormal body size (shorter and wider compared to controls) at the second instar larval stage. Tb1 homozygous larvae also exhibit electron-dense aggregates immersed within the procuticle; epidermal cells which are shorter along the anterior posterior axis and longer along the dorsoventral axis of the animal; and the inner cuticular surface of larvae that form convexities but are comparably flat with random orientation.

Tb1 larvae are considerably shorter along the anterior-posterior axis than wild-type. Although the length of the Tb1 ventral nerve cord is comparable to wild-type, peripheral nerves are shorter in Tb1 larvae.

Heterozygous Tb1 mutant larvae have short bodies and long tracheal tubes compared to wild type.

The intertaenidial distance in Tb1 heterozygotes is similar to wild type.

Tb1 mutant pupae exhibit a reduced cuticle axial ratio (length\width), without any defects in viability or fertility. At the pupal stage Tb1 mutants exhibit an axial ratio of 2.0 (compared to 3.5 in wild-type).

Larvae, pupae and adults are shorter and thicker than wild-type in both heterozygotes and homozygotes (not separable). Mutant larvae are distinguishable from wild-type on the basis of reduced length and tortuous tracheal trunks. Classification is reliable in larvae and pupae but not adults.

Larvae, pupae and adult are short and thickset. RK1 from first instar through pupa, RK2 as adult.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference

Tb+/Tb1 is a suppressor | partially of lethal | larval stage phenotype of Mmp12

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
NOT Suppressor of
Statement
Reference

Tb+/Tb1 is a non-suppressor of taenidium | larval stage phenotype of Mmp12

Additional Comments
Genetic Interactions
Statement
Reference

A Tb1 heterozygous background suppresses the ventral nerve cord extension found in GlcAT-Pbrv1 homozygous mutant L3 larvae, however, these double mutants still exhibit extension compared to wild-type controls. This demonstrates that the ventral nerve cord extension correlates with the total length of the larva, suggesting that in GlcAT-Pbrv1 mutants the ventral nerve cord expands as a consequence of increased tension from shorter peripheral nerves or trachea, possibly due to some growth retardation of larval peripheral nerves or trachea.

One copy of Tb1 in a Mmp12 mutant background partially rescues the tracheal break phenotype seen in Mmp12 homozygotes (55% of larva have tracheal breaks compared to 93%).

The angle between the transverse connective and the dorsal trunk is reduced in Tb1 Mmp12 double mutants compared to homozygous Mmp12 mutants but is still greater than in wild type.

No homozygous Mmp12 larvae survive to pupariation, whereas 29% of Tb1 Mmp12 double mutants survive beyond the larval stages.

The failure of the taenidial distance to increase from the early to late third instar stages that is seen in Mmp12 mutants is still present in flies that also carry one copy of Tb1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (5,172)
Notes on Origin
Discoverer

Auerbach.

Comments
Comments

"nitrogen mustard" was stated as tentative.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (20)