FlyBase Allele Report: Dmel\Tb[1]

General Information

Symbol

Dmel\Tb¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016730

Feature type

allele

Associated gene

Dmel\Tb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

nitrogen mustard

Nature of the Allele

Allele class

Mutagen

nitrogen mustard

(Craymer, 1980)

Progenitor genotype

Cytology

Description

In-frame 15bp deletion in the second exon (coordinates 3R:26656728..26656742 , release 6 genome), 69bp in-frame deletion of 23 amino acid residues ( 3R:26657089..26657157 ) and a T to G (Ser to Ala) mutation at 3R:26657334 .

(Miller et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:26,656,728..26,656,742 [+]

Comment:

An in-frame 15 bp deletion in Tb. One of three mapped lesions in Tb in mutant.

(Miller et al., 2016)

deletion

3R:26,657,089..26,657,157 [+]

Comment:

69bp in-frame deletion of 23 amino acid residues. One of three mapped lesions in Tb in Tb¹ mutant strain.

(Miller et al., 2016)

point_mutation

3R:26,657,334..26,657,334 [+]

Nucleotide change:

T26657334G

Reported nucleotide change:

T/G

Amino acid change:

S249A | Tb-PA

Reported amino acid change:

S?A

Comment:

Missense mutation in Tb. One of three mapped lesions in Tb in mutant.

(Miller et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult

(Lindsley and Zimm, 1992)

embryonic/larval tracheal dorsal trunk

(Glasheen et al., 2010)

epidermal cell | larval stage

(Zuber et al., 2020)

larva

(Lindsley and Zimm, 1992)

larval peripheral nervous system

(Pandey et al., 2011)

larval tracheal system

(Lindsley and Zimm, 1992)

procuticle | larval stage

(Zuber et al., 2020)

pupa

(Lindsley and Zimm, 1992)

Detailed Description

Statement

Reference

Tb¹ homozygous larvae start to exhibit abnormal body size (shorter and wider compared to controls) at the second instar larval stage. Tb¹ homozygous larvae also exhibit electron-dense aggregates immersed within the procuticle; epidermal cells which are shorter along the anterior posterior axis and longer along the dorsoventral axis of the animal; and the inner cuticular surface of larvae that form convexities but are comparably flat with random orientation.

(Zuber et al., 2020)

Tb¹ larvae are considerably shorter along the anterior-posterior axis than wild-type. Although the length of the Tb¹ ventral nerve cord is comparable to wild-type, peripheral nerves are shorter in Tb¹ larvae.

(Pandey et al., 2011)

Heterozygous Tb¹ mutant larvae have short bodies and long tracheal tubes compared to wild type.

The intertaenidial distance in Tb¹ heterozygotes is similar to wild type.

(Glasheen et al., 2010)

Tb¹ mutant pupae exhibit a reduced cuticle axial ratio (length\width), without any defects in viability or fertility. At the pupal stage Tb¹ mutants exhibit an axial ratio of 2.0 (compared to 3.5 in wild-type).

(Guan et al., 2006)

Larvae, pupae and adults are shorter and thicker than wild-type in both heterozygotes and homozygotes (not separable). Mutant larvae are distinguishable from wild-type on the basis of reduced length and tortuous tracheal trunks. Classification is reliable in larvae and pupae but not adults.

(Lindsley and Zimm, 1992)

Larvae, pupae and adult are short and thickset. RK1 from first instar through pupa, RK2 as adult.

(Craymer, 1980)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Tb¹ has abnormal body size | larval stage phenotype, non-enhanceable by TwdlL^KK113269/Scer\GAL4^e22c

(Zuber et al., 2020)

NOT suppressed by

Statement

Reference

Tb¹ has abnormal body size | larval stage phenotype, non-suppressible by TwdlL^KK113269/Scer\GAL4^e22c

(Zuber et al., 2020)

Suppressor of

Statement

Reference

Tb[+]/Tb¹ is a suppressor | partially of abnormal neuroanatomy phenotype of GlcAT-P^brv1

(Pandey et al., 2011)

Tb[+]/Tb¹ is a suppressor of lethal | larval stage phenotype of Mmp1²

(Glasheen et al., 2010)

NOT Suppressor of

Statement

Reference

Tb[+]/Tb¹ is a non-suppressor of abnormal size | late third instar larval stage phenotype of Mmp1²

(Glasheen et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Tb[+]/Tb¹ is a suppressor | partially of larval ventral nerve cord | larval stage phenotype of GlcAT-P^brv1

(Pandey et al., 2011)

Tb[+]/Tb¹ is a suppressor | partially of larval peripheral nervous system phenotype of GlcAT-P^brv1

(Pandey et al., 2011)

Tb[+]/Tb¹ is a suppressor of embryonic/larval tracheal dorsal trunk phenotype of Mmp1²

(Glasheen et al., 2010)

NOT Suppressor of

Statement

Reference

Tb[+]/Tb¹ is a non-suppressor of taenidium | larval stage phenotype of Mmp1²

(Glasheen et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

A Tb¹ heterozygous background suppresses the ventral nerve cord extension found in GlcAT-P^brv1 homozygous mutant L3 larvae, however, these double mutants still exhibit extension compared to wild-type controls. This demonstrates that the ventral nerve cord extension correlates with the total length of the larva, suggesting that in GlcAT-P^brv1 mutants the ventral nerve cord expands as a consequence of increased tension from shorter peripheral nerves or trachea, possibly due to some growth retardation of larval peripheral nerves or trachea.

(Pandey et al., 2011)

One copy of Tb¹ in a Mmp1² mutant background partially rescues the tracheal break phenotype seen in Mmp1² homozygotes (55% of larva have tracheal breaks compared to 93%).

The angle between the transverse connective and the dorsal trunk is reduced in Tb¹ Mmp1² double mutants compared to homozygous Mmp1² mutants but is still greater than in wild type.

No homozygous Mmp1² larvae survive to pupariation, whereas 29% of Tb¹ Mmp1² double mutants survive beyond the larval stages.

The failure of the taenidial distance to increase from the early to late third instar stages that is seen in Mmp1² mutants is still present in flies that also carry one copy of Tb¹.

(Glasheen et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (6,657)

Bloomington

624