Tlr3/Tlrv1 flies do not exhibit significantly different survival rates in response to Staphylococcus aureus infection, as compared to control flies.
Tlr3/Tlrv1 flies infected with Pythium insidiosum show significantly lower survival rates than wild-type. As evidenced by histopathological studies, injected P. insidiosum zoospores germinate rapidly in fly hemolymph to form hyphae that subsequently disseminate and invade the thorax, abdomen and head of the mutants. No histopathological alterations are found in wild-type flies after P. insidiosum infection.
Mutant flies show no significant difference in mortality compared to wild-type flies after infection with either Providencia rettgeri or Providencia burhodogranariea.
Tlr3/Tlrv1 flies show a reduced survival rate compared to control flies after infection with either S. aureus or A. fumigatus.
Infection with the S. aureus 'ItaS' mutant causes earlier death than infection with its parent RN4220 strain in Tlrv1/Tlr3 flies, similar to the findings with wild type flies.
Adult females transheterozygous for Tlr3/Tlrv1 display increased sensitivity to infection with various strains of Candida albicans: the survival rate of infected flies is significantly decreased compared to wild-type. Tlr3/Tlrv1 also show significantly higher post-infection fungal load, which unlike in wild-type flies progressively increases with time when infected with a wild-type strain of C. albicans.
The ability of Tlr2/Tlrv1 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.
The concentration of circulating hemocytes in Tlrv1/Tlr3 larvae is reduced compared to controls.
Level of Drs induction of bacterially challenged Tlr3/Tlrv1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Inducibility of all antimicrobial genes by bacterial challenge in imd1/imd1; Tlr3/Tlrv1 double mutants is severely reduced. Septic injury (pricking with a needle under nonsterile conditions) or infection with E.coli does not noticeably affect Tlr3/Tlrv1 survival, infection with A.fumigatus results in death after 2-3 days clearly associated with uncontrolled fungal development. 40% homozygous double mutant flies survive after septic injury but only a few individuals survive 3 days postinfection with E.coli. Infection of Tlr3/Tlrv1 mutants with A.fumigatus causes 8% survival 3 days postinfection, infection with E.coli causes survival rates similar to wild type.