FlyBase Allele Report: Dmel\trol[8]

General Information

Symbol

Dmel\trol⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0017053

Feature type

allele

Associated gene

Dmel\trol

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

N-methyl-N'-nitro-N-nitrosoguanidine

Nature of the Allele

Allele class

Mutagen

N-methyl-N'-nitro-N-nitrosoguanidine

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Skeath et al., 2017)

abnormal neuroanatomy | embryonic stage

(Cho et al., 2012)

abnormal neuroanatomy | larval stage

(Skeath et al., 2017)

lethal | recessive

(Judd et al., 1972)

Phenotype Manifest In

larval brain | third instar larval stage

(Skeath et al., 2017)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Cho et al., 2012)

larval segmental nerve branch SNa of A1-7 | embryonic stage

(Cho et al., 2012)

larval ventral nerve cord | larval stage

(Skeath et al., 2017)

Detailed Description

Statement

Reference

trol⁸ mutant larvae display morphological defects in the brain: a neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures) as well as protrusions of varied size from the brain and nerve cord, however, the penetrance is relatively low as the larvae typically arrest in the second instar larval stage.

(Skeath et al., 2017)

Hemizygous embryos show ISNb motor axon guidance defects (69.1% of hemisegments) and SNa motor axon guidance defects (41.1% of hemisegments). ISNb axons fail to defasciculate at muscles 6 and 7 and some axons stall between muscles 6 and 13. SNa axons fail to reach their proper targets.

(Cho et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

trol[+]/trol⁸ is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

Sema1a^k13702, trol[+]/trol⁸ has abnormal neuroanatomy | dominant | embryonic stage phenotype

(Cho et al., 2012)

Df(4)C3/+, trol⁸ has abnormal neuroanatomy | dominant | embryonic stage phenotype

(Cho et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

trol[+]/trol⁸ is a suppressor of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Other

Statement

Reference

Sema1a^k13702, trol[+]/trol⁸ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Sema1a^k13702, trol[+]/trol⁸ has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Df(4)C3/+, trol⁸ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Df(4)C3/+, trol⁸ has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype

(Cho et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background is significantly suppressed if the embryos are also heterozygous for trol⁸.

trol⁸/+ ; Sema-1a^k13702/+ double heterozygous embryos show ISNb motor axon guidance defects (50.5% of hemisegments) and SNa motor axon guidance defects (44.8% of hemisegments).

trol⁸/+ ; Df(4)C3/+ double heterozygous embryos show ISNb motor axon guidance defects (60.2% of hemisegments) and SNa motor axon guidance defects (52.4% of hemisegments).

trol⁸/+ ; plexB^KG00878/+ double heterozygous embryo do not show significant ISNb or SNa motor axon guidance defects compared to controls.

(Cho et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Kaufman.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

l(1)3Ac⁸

l(1)zw1^45a

(Judd et al., 1972)

trol⁸

(Skeath et al., 2017, Cho et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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