FlyBase Allele Report: Dmel\usp[4]

General Information

Symbol

Dmel\usp⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0017653

Feature type

allele

Associated gene

Dmel\usp

Associated Insertion(s)

Carried in Construct

Also Known As

usp^VE849

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zelhof et al., 1997)

Mutagen

ethyl methanesulfonate

(Perrimon et al., 1984)

chemical

(Oro et al., 1992)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Oro et al., 1992)

Description

Amino acid replacement: R?C. The amino acid replacement is at a phosphate contacting Arginine residue within the second zinc finger of the DNA binding domain.

(Lee et al., 2000)

Missense mutation in the DNA-binding domain.

(Zelhof et al., 1997)

Nucleotide substitution: C?T. Amino acid replacement: R130C. Mutation is in the conserved arginine residues in the DNA binding domain (DBD).

(Henrich et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,040,526..2,040,526 [-]

Nucleotide change:

C2040526T

Amino acid change:

R130C | usp-PA; R130C | usp-PB

(Henrich et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Henrich et al., 1994)

lethal | recessive

(Oro et al., 1990, Perrimon et al., 1985)

Phenotype Manifest In

egg chamber | germline clone

(Perrimon et al., 1984)

embryonic/first instar larval cuticle | germline clone

(Oro et al., 1992)

eye disc morphogenetic furrow | somatic clone

(Zelhof et al., 1997)

ommatidial precursor cluster | somatic clone

(Zelhof et al., 1997)

Detailed Description

Statement

Reference

Expression of usp^hs.PO using a single 30 minute 37^oC heat pulse during the first larval instar stage is sufficient to rescue more than 70% of usp⁴ animals to the third instar stage. These rescued animals often wander only a short distance from the food near the end of the third instar stage.

(Hall and Thummel, 1998)

Homozygous clones in the eye imaginal disc that lie posterior to the morphogenetic furrow have an abnormal arrangement of developing photoreceptor clusters. The clusters are irregularly spaced and differentiate prematurely, although they contain the normal number of cells. Movement of the morphogenetic furrow is accelerated where it passes through a homozygous clone. This displacement of the furrow is not caused by excess proliferation of usp⁴ cells in the clone.

(Zelhof et al., 1997)

Heterozygotes display thoracic defects ranging from mild, slight separation of the microchaetes and macrochaetes along the dorsal midline, to severe, a cleft extending through both the notum and scutellum. Flies also have bent and misshapen sensory bristles and develop severely knarled legs.

(Henrich et al., 1994)

Females with usp mutant germ cells give rise to fully viable and fertile female usp mutant heterozygotes, but non-viable usp mutant/Y males. Most of these males die just prior to hatching, with the rest dying shortly after. The unhatched embryos have cuticular scarring in the region posterior of A9. In contrast usp mutant/Y males derived from germ cells with functional usp hatch and die between the first and second instar periods, with no gross cuticular defects. usp⁴ mosaic females mutant in the germline lay very few unfertilised eggs, in contrast to usp⁴ homozygous females (mutant both soma and germline), indicating that the fertilisation defect in these homozygotes is due to lack of usp in the soma.

(Oro et al., 1992)

Lethality occurs during embryonic and larval stages. Phenotype of homozygous germ line clones is maternal effect lethal.

(Perrimon et al., 1985)

Homozygous usp⁴ germline generates misshapen eggs.

(Perrimon et al., 1984)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

usp⁴ has lethal | larval stage phenotype, suppressible by usp::Cten\Usp-1^d-c

(Henrich et al., 2000)

Other

Statement

Reference

Df(2L)let-7-C^KO1, P{W8,let7C^Δmir-let7}, usp⁴ has abnormal neuroanatomy phenotype

(Kucherenko et al., 2012)

EcR[+]/EcR^Q50st, usp⁴ has abnormal neuroanatomy phenotype

(Kucherenko et al., 2012)

Scer\GAL4^αTub84B.PL, ari-1^UAS.cFa, usp⁴/usp[+] has lethal | embryonic stage phenotype

(Gradilla et al., 2011)

Scer\GAL4^αTub84B.PL, ari-1³⁷⁴⁷, ari-1^UAS.cFa, usp⁴/usp[+] has lethal | second instar larval stage phenotype

(Gradilla et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

Df(2L)let-7-C^KO1, P{W8,let7C^Δmir-let7}, usp⁴ has lobe system of adult mushroom body phenotype

(Kucherenko et al., 2012)

EcR[+]/EcR^Q50st, usp⁴ has lobe system of adult mushroom body phenotype

(Kucherenko et al., 2012)

EcR[+]/EcR^Q50st, usp⁴ has adult mushroom body beta-lobe phenotype

(Kucherenko et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

usp⁴/+ ; Df(2L)let-7-C^KO1/+ ; P{W8,let-7-C^Δlet-7} animals have defects in the mushroom body α/β lobes: the lobes may appear slim.

usp⁴/+ ; EcR^Q50st/+ animals have defects in the mushroom body α/β lobes: the lobes may appear slim or the β lobes may be fused.

(Kucherenko et al., 2012)

Xenogenetic Interactions

Statement

Reference

Mutant larvae rescued by one copy of usp::Cten\Usp-1^d-c die at the late third instar stage. Rescued usp⁴ mutants show a cessation of movement during the late third instar, but larvae show no sign of prepupal tanning except along the denticle belts of the midsegments. The addition of a second copy of usp::Cten\Usp-1^d-c leads to further tanning of larvae, but they continue to fail to undergo pupal contraction, and anterior spiracle eversion is incomplete. The addition of three and four copies does allow some adults to survive.

(Henrich et al., 2000)

Complementation and Rescue Data

Rescued by

usp⁴ is rescued by usp^tOa

(Zelhof et al., 1997)

usp⁴ is rescued by usp^tOa

(Oro et al., 1990)

Not rescued by

usp⁴ is not rescued by usp^GMR.PZ

(Zelhof et al., 1997)

Comments

The usp⁴ eye imaginal disc phenotype is rescued by usp^tOa but not by usp^GMR.PZ.

(Zelhof et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (2)

4660

107830

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

VE849

(Perrimon et al., 1985)

l(1)VE849

usp⁴

(Kucherenko et al., 2012, Boulanger et al., 2011, Gradilla et al., 2011, König et al., 2011, Chavoshi et al., 2010, Popodi et al., 2010-)

usp^VE849

(Oro et al., 1992, Oro et al., 1990)

Name Synonyms

Secondary FlyBase IDs

References (20)

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