FlyBase Allele Report: Dmel\grk[HF]

General Information

Symbol

Dmel\grk^HF

Species

D. melanogaster

Name

FlyBase ID

FBal0031411

Feature type

allele

Associated gene

Dmel\grk

Associated Insertion(s)

Carried in Construct

Also Known As

grk^HF48

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neuman-Silberberg and Schupbach, 1996, Neuman-Silberberg and Schupbach, 1993, Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C1721T.

(Thio et al., 2000)

Amino acid replacement: Q56term.

(Thio et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,432,396..8,432,396 [-]

Nucleotide change:

C8432396T

Reported nucleotide change:

C1721T

Amino acid change:

Q57term | grk-PA

Reported amino acid change:

Q56term

(Thio et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Neuman-Silberberg and Schupbach, 1993, Schupbach and Wieschaus, 1991)

some die during embryonic stage (with grk^ΔFRT), with grk^ss-mut

(Rathore et al., 2020)

some die during first instar larval stage (with grk^ΔFRT), with grk^ss-mut

(Rathore et al., 2020)

sterile (with grk^ΔFRT)

(Rathore et al., 2020)

Phenotype Manifest In

dorsal appendage

(Chang et al., 2008, Schupbach and Wieschaus, 1991)

dorsal appendage (with grk^ΔFRT)

(Rathore et al., 2020)

dorsal appendage | cold sensitive

(Doronkin et al., 2002)

egg

(Chang et al., 2008)

egg operculum (with grk^2B6)

(Shravage et al., 2007)

micropyle

(Schupbach and Wieschaus, 1991)

Detailed Description

Statement

Reference

grk^HF/+ mothers lay mildly ventralised eggs (about 54%). grk^HF homozygous mothers lay 100% of eggs with strong ventralised egg shells.

(Chang et al., 2008)

Eggs derived from grk^2B6/grk^HF females lack operculum regions II and III (the section that normally extends from the area surrounding the micropyle to the dorsal appendages and the section that is normally between the dorsal appendages), and instead only region I cells are present symmetrically surrounding the micropyle, following a symmetric collar that separates the operculum from the rest of the chorion.

(Shravage et al., 2007)

Heterozygous females lay eggs with fused or partially fused dorsal appendages (98% at 18^oC, 66.7% at 25^oC and 34% at 29^oC).

(Doronkin et al., 2002)

Embryos exhibit reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle is present at both ends of eggs, embryos are ventralized.

(Schupbach and Wieschaus, 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

grk^HF has dorsal appendage phenotype, enhanceable by CSN5^L4032/CSN5[+]

(Doronkin et al., 2002)

NOT Enhancer of

Statement

Reference

grk^HF is a non-enhancer of ventral denticle belt phenotype of Krn²⁷, spi¹, vn^L6

(Austin et al., 2014)

Other

Statement

Reference

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has egg chorion phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has egg phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has micropyle phenotype

(Shravage et al., 2007)

Pvf1^EP1624, grk^HF, spi¹ has egg chamber phenotype

(McDonald et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

vn^L6 enhances the ventral patterning defects seen in spi¹ mutant embryos. The double mutants have a reduced number of denticle belts compared to controls, fusion of the denticle belts and an anterior hole. The phenotype is further exacerbated by Krn²⁷, with embryos having no denticle belts and a large anterior hole. A zygotic grk^HF mutation has no additional effect.

Krn²⁷ enhances the ventral patterning defects seen in spi¹ mutant embryos. The double mutants have a reduced number of denticle belts compared to controls, fusion of the denticle belts and an anterior hole. The phenotype is further exacerbated by vn^L6, with embryos having no denticle belts and a large anterior hole. A zygotic grk^HF mutation has no additional effect.

(Austin et al., 2014)

Eggs derived from females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^CY2 in a grk^2B6/grk^HF background have misshaped micropyles, show the loss of the collar that normally separates the operculum from the rest of the chorion and show a reduction in egg diameter.

(Shravage et al., 2007)

Expression of grk::yl^{grk1-275.yl1826-1984} is able to suppress the grk^2B6/grk^HF oogenesis phenotype.

grk::yl^{grk1-275.yl1826-1984}.

(Bokel et al., 2006)

The egg chambers of Pvf1^EP1624; spi¹; grk^HF females do not develop normally.

(McDonald et al., 2006)

The fused dorsal appendage phenotype of eggs laid by grk^HF/+ females is enhanced if the females are also heterozygous for CSN5^L4032.

(Doronkin et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

grk^HF/grk^ΔFRT is rescued by grk^+tRa

(Rathore et al., 2020)

grk^HF/grk^ΔFRT is rescued by grk^no-int1

(Rathore et al., 2020)

grk^HF is rescued by grk^HRP

(Chang et al., 2008)

Partially rescued by

grk^HF/grk^ΔFRT is partially rescued by grk^ss-mut

(Rathore et al., 2020)

Comments

grk^T:Arus\HRP rescues the ventralised egg shell phenotype of eggs laid by grk^HF mutant mothers.

(Chang et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

grk^HF48

(Technau et al., 2012, Bokel et al., 2006, Lin et al., 2006, Schnorrer et al., 2000, Thio et al., 2000, Schupbach and Wieschaus, 1991)

grk^HF

(Rathore et al., 2020, Austin et al., 2014, Tsai et al., 2014, Dubin-Bar et al., 2011, Becalska and Gavis, 2010, Liu et al., 2010, Chang et al., 2008, Duong et al., 2008, Shravage et al., 2007, McDonald et al., 2006)

Name Synonyms

Secondary FlyBase IDs

FBal0031410

References (20)

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