FlyBase Allele Report: Dmel\peb[X001]

General Information

Symbol

Dmel\peb^X001

Species

D. melanogaster

Name

FlyBase ID

FBal0031500

Feature type

allele

Associated gene

Dmel\peb

Associated Insertion(s)

Carried in Construct

Also Known As

hnt^XO01

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: Q348term.

(Yip et al., 1997)

The truncated protein produced is predicted to contain only 3 of the 14 zinc fingers present in the wild-type protein.

(Yip et al., 1997)

Nucleotide substitution: C?T.

(Yip et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,621,326..4,621,326 [+]

Nucleotide change:

C4621326T

Reported nucleotide change:

C?T

Amino acid change:

Q348term | peb-PA; Q348term | peb-PB

Reported amino acid change:

Q348term

(Yip et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Yip et al., 1997, Perrimon et al., 1989)

some die during embryonic stage | recessive

(Yip et al., 1997, Perrimon et al., 1989)

visible | somatic clone

(Pickup et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

R3/R3 type symmetrical ommatidia (where the R3 or R4 cell has not taken up the correct position) are found at the borders of homozygous clones in the eye. In these ommatidia, either the R3 or R4 cell, or both, is mutant and the chirality of these ommatidia is sometimes also incorrect. Homozygous clones in the eye result in a scar. Mutant cells in larval eye discs show depletion or more diffuse localisation of F-actin, especially in the more posterior part of the mutant clone.

(Pickup et al., 2002)

84.4% of embryos exhibit a germband retraction phenotype, 12.3% a dorsal closure phenotype. 79% of embryos that complete germ band retraction exhibit an anterior-open or dorsal-hole phenotype characteristic of the failure of dorsal closure.

(Reed et al., 2001)

Genetic mosaic embryos show phenotypes that reveal a correlation between the genotype of the amnioserosa and the success or failure of germ band retraction.

(Lamka and Lipshitz, 1999)

Hemizygous embryos show weak failure of germband retraction. 68% have abdominal segment 6.5 or greater at the posterior tip. The head skeleton is abnormal.

(Yip et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

peb^X001 has amnioserosa phenotype, non-suppressible by InR^hs.PF

(Lamka and Lipshitz, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Blocking amnioserosal cell death by making the mutant embryos also mutant for Df(3L)H99 does not alter the effect of mutant clones. InR^hs.PF almost completely suppresses the germ band retraction defect of peb^X001 embryos. The premature apoptosis of amnioserosa cells is not, however, rescued.

(Lamka and Lipshitz, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

peb¹

(Pickup et al., 2002)

peb¹

(Yip et al., 1997)

Rescued by

peb^X001 is rescued by peb^hs.PY

(Yip et al., 1997)

Comments

The failure of germband retraction can be rescued by peb^hs.PY.

(Yip et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Maternal germline clonal analysis demonstrates there is no maternal effect.

(Perrimon et al., 1989)

l(1)EH275a is not allelic to peb; l(1)EH275a¹ complements peb^hnt-1, peb^X001, peb^EH704a and peb¹.

(Yip et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hnt^XO01

(Pickup et al., 2002, Reed et al., 2001, Lamka and Lipshitz, 1999)

peb^X001

Name Synonyms

Secondary FlyBase IDs

References (5)

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