FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mei-S3324
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General Information
Symbol
Dmel\mei-S3324
Species
D. melanogaster
Name
FlyBase ID
FBal0032117
Feature type
allele
Associated gene
Dmel\mei-S332
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Kerrebrock et al., 1995, Kerrebrock et al., 1992)
Progenitor genotype
Cytology
Description

Amino acid replacement: L112M. Amino acid replacement: P377H.

(Kerrebrock et al., 1995)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:22,123,331..22,123,331 [+]
Nucleotide change:

T22123331A

Amino acid change:

L112M | mei-S332-PA; L112M | mei-S332-PB

Reported amino acid change:

L112M

Comment:

First of two amino acid changes in mutant.

(Kerrebrock et al., 1995)
point_mutation
2R:22,124,188..22,124,188 [+]
Nucleotide change:

C22124188A

Amino acid change:

P377H | mei-S332-PA; P377H | mei-S332-PB

Comment:

Second of two amino acid changes in mutant.

(Kerrebrock et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In mei-S3324/mei-S3327 mutants, loss of sister-chromatid cohesion results in male gametes lacking sex chromosomes or containing two sister X chromosomes. Spermatozoa with X and Y chromosome, indicative of meiosis I non-disjunction, occur at low frequency.

      (Nogueira et al., 2014)

      High levels of nondisjunction in males and females.

      (Kerrebrock et al., 1995)

      Defects in sister chromatid cohesion. 20--30% total non-disjunction, hemizygotes exhibited a small increase in total nondisjunction. High frequency of premature separation of sister chromatids in prophase II. Nondisjunction of chromosomes 2 and 3 in males and females.

      (Kerrebrock et al., 1992)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mei-S3327/mei-S3324 is rescued by mei-S332GFP

      (Nogueira et al., 2014)

      mei-S3327/mei-S3324 is rescued by mei-S332S124,5,6-A.GFP

      (Nogueira et al., 2014)

      mei-S3327/mei-S3324 is rescued by mei-S332T331-A.GFP

      (Nogueira et al., 2014)

      mei-S3327/mei-S3324 is rescued by mei-S332T331-D.GFP

      (Nogueira et al., 2014)
      Comments

      Expression of mei-S332T:Avic\GFP rescues the meiotic chromosome segregation defects of mei-S3324/mei-S3327 mutants.

      Expression of mei-S332S124,5,6-A.T:Avic\GFP rescues the meiotic chromosome segregation defects of mei-S3324/mei-S3327 mutants.

      Expression of mei-S332T331-A.T:Avic\GFP rescues the meiotic chromosome segregation defects of mei-S3324/mei-S3327 mutants.

      Expression of mei-S332T331-D.T:Avic\GFP rescues the meiotic chromosome segregation defects of mei-S3324/mei-S3327 mutants.

      (Nogueira et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Strong mei-S332 allele.

      (Kerrebrock et al., 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      mei-S3324
      (Jang et al., 2021, Nogueira et al., 2014, Yan et al., 2010, Resnick et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)