FlyBase Allele Report: Dmel\mei-S332[7]

General Information

Symbol

Dmel\mei-S332⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0032120

Feature type

allele

Associated gene

Dmel\mei-S332

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Kerrebrock et al., 1995, Kerrebrock et al., 1992)

Mutagen

ethyl methanesulfonate

(Kerrebrock et al., 1995, Kerrebrock et al., 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: ??term.

(Moore et al., 1998)

The premature stop codon is predicted to result in a truncated protein that lacks the C-terminal 109 amino acids of the wild-type product.

(Kerrebrock et al., 1995)

Amino acid replacement: R293term.

(Kerrebrock et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:22,123,935..22,123,935 [+]

Nucleotide change:

C22123935T

Amino acid change:

R293term | mei-S332-PA; R293term | mei-S332-PB

Reported amino acid change:

R293term

(Kerrebrock et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Williams et al., 1996)

abnormal meiotic cell cycle | recessive

(Tang et al., 1998, Kerrebrock et al., 1992)

abnormal mitotic cell cycle

(Kerrebrock et al., 1995)

male sterile

(Kerrebrock et al., 1992)

Phenotype Manifest In

meiosis II | female

(Tang et al., 1998)

meiosis II | male

(Tang et al., 1998)

neuroblast & mitotic cell cycle (with Df(2R)X58-6)

(LeBlanc et al., 1999)

sex chromosome (with mei-S332⁴)

(Nogueira et al., 2014)

spermatozoon (with mei-S332⁴)

(Nogueira et al., 2014)

Detailed Description

Statement

Reference

In mei-S332⁴/mei-S332⁷ mutants, loss of sister-chromatid cohesion results in male gametes lacking sex chromosomes or containing two sister X chromosomes. Spermatozoa with X and Y chromosome, indicative of meiosis I non-disjunction, occur at low frequency.

(Nogueira et al., 2014)

Transmission of minichromosomes is decreased in mei-S332⁷ mutants. Transmission frequencies decline significantly in the derivatives in which core components of the centromere are deleted.

(Lopez et al., 2000)

Neuroblasts in mei-S332⁷/Df(2R)X58-6 mutants show a normal mitotic index but increased anaphase frequency. 17% of prometaphase cells exhibit precocious separation of sister chromatids, compared to 10% in wild-type.

(LeBlanc et al., 1999)

Hemizygous flies show high frequencies of chromosome loss and mis-segregation in both male and female meiosis II.

(Tang et al., 1998)

Precocious sister chromatid separation (PSCS) during anaphase I, resulting almost exclusively in second-division missegregation.

(Williams et al., 1996)

High levels of nondisjunction in males and females.

(Kerrebrock et al., 1995)

Defects in sister chromatid cohesion. 20--30% total non-disjunction, hemizygotes exhibited a small increase in total nondisjunction. High frequency of premature separation of sister chromatids in prophase II. Nondisjunction of chromosomes 2 and 3 in males and females.

(Kerrebrock et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions