FlyBase Allele Report: Dmel\Raf[raf-1]

General Information

Symbol

Dmel\Raf^raf-1

Species

D. melanogaster

Name

FlyBase ID

FBal0032422

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

Also Known As

D-raf¹, l(1)raf¹

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

nitrosomethyl urea

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hata et al., 1994, Tsuda et al., 1993)

Mutagen

nitrosomethyl urea

(Nishida et al., 1988)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q412term.

(Radke et al., 1997, Hata et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,342,119..2,342,119 [+]

Nucleotide change:

C2342119T

Amino acid change:

Q369term | Raf-PA; Q369term | Raf-PE

Reported amino acid change:

Q412term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lu et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | embryonic stage | somatic clone

(Tsuda et al., 1993)

lethal | recessive

(Nishida et al., 1988)

Phenotype Manifest In

embryo | posterior

(Radke et al., 1997)

Detailed Description

Statement

Reference

Homozygous embryos exhibit defects in posterior pattern.

(Radke et al., 1997)

Humeral disc cells of phl^raf-1 embryos are reduced in number and arrested at the G2/M boundary.

(Hayashi, 1996)

No aberrant mitotic figures are observed at 28^oC.

(Hata et al., 1994)

Humeral discs of phl^raf-1 flies show reduced number of imaginal cells, while size of nuclei remains normal. Humeral discs of esg⁰⁵⁷²⁹/esg^35Ce-3 flies have nuclei similar in size to wild type, and differentiate normal dorsal T1 structures. Combination of phl^raf-1 with esg⁰⁵⁷²⁹/esg^35Ce-3 greatly increased size of the imaginal cell nuclei.

(Hayashi et al., 1993)

Twin spot analysis demonstrates a 38% reduction of the proliferation rate. The proliferation rate is significantly recovered by Dsor1^Su1.

(Tsuda et al., 1993)

Lethal phase is either at larval or pupal stages. The proliferating cells of both somatic and germ line origins are severely affected. The construct P{C20-Draf} confers partial rescue of the lethal phenotype.

(Nishida et al., 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Raf^raf-1 has decreased occurrence of cell division | somatic clone | embryonic stage phenotype, suppressible by Dsor1^Su1

(Tsuda et al., 1993)

Phenotype Manifest In

Suppressor of

Statement

Reference

Raf^raf-1/phl[+] is a suppressor of phenotype of aos²⁵⁷

(Sawamoto et al., 1996)

Raf^raf-1 is a suppressor of phenotype of Src42A^KR.hs.2sev

(Takahashi et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Twin spot analysis demonstrates a 38% reduction of the proliferation rate. The proliferation rate is significantly recovered by Dsor1^Su1.

(Tsuda et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Injection of wild type RNA rescues the filzkorper, tuft and anal pads.

(Radke et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Y. Nishida.

(Hayashi et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

D-raf¹

(Hayashi, 1996, Sawamoto et al., 1996, Takahashi et al., 1996, Hata et al., 1994)

D-raf^raf1

(Radke et al., 1997)

I-2-6-1

Raf^raf-1

l(1)raf¹

(Tsuda et al., 1993, Nishida et al., 1988)

phl^raf-1

raf¹

(Hayashi et al., 1993)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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