This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
The level of SmD3 transcript is strongly reduced in P{lacW}SmD3k09037 homozygotes compared to wild type, whereas Oda expression remains essentially unaffected. The P{lacW}SmD3k09037 insertion appears to affect only SmD3 function and not Oda function (despite being within an Oda intron), since the lethality of P{lacW}SmD3k09037 homozygotes is fully rescued by the P{SmD3.+t2.3} construct which contains the complete SmD3 gene.
This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
The level of SmD3 transcript is strongly reduced in P{lacW}SmD3k09037 homozygotes compared to wild type, whereas Oda expression remains essentially unaffected. The P{lacW}SmD3k09037 insertion appears to affect only SmD3 function and not Oda function (despite being within an Oda intron), since the lethality of P{lacW}SmD3k09037 homozygotes is fully rescued by the P{SmD3.+t2.3} construct which contains the complete SmD3 gene.