FlyBase Allele Report: Dmel\mys[hs.PB]

General Information

Symbol

Dmel\mys^hs.PB

Species

D. melanogaster

Name

FlyBase ID

FBal0035243

Feature type

allele

Associated gene

Dmel\mys

Associated Insertion(s)

Carried in Construct

P{mys⁺hs}

Key Links

Transgenic product class

cDNA

(Bunch et al., 1992)

wild_type

(Bunch et al., 1992)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bunch et al., 1992)

wild_type

(Bunch et al., 1992)

Mutagen

in vitro construct

(Brabant and Brower, 1993, Bunch et al., 1992)

Progenitor genotype

Carried in construct

P{mys⁺hs}

Cytology

Description

An Hsp70 promoter drives expression of a mys cDNA.

(Bunch et al., 1992)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

mys

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | heat sensitive

(Beumer et al., 1999)

lethal | heat sensitive

(Beumer et al., 1999)

wild-type

(Ayyub et al., 2000, Longley and Ready, 1995, Brabant and Brower, 1993, Bunch et al., 1992)

Phenotype Manifest In

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1 | conditional ts

(Beumer et al., 1999)

bouton & neuromuscular junction | conditional ts

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 1 | conditional ts

(Beumer et al., 1999)

neuromuscular junction | heat sensitive

(Beumer et al., 1999)

Detailed Description

Statement

Reference

Ectopic expression of mys^hs.PB has no effect on olfactory function.

(Ayyub et al., 2000)

Overexpression of mys^hs.PB using heat shock every 7 hours from hatching results in larval and pupal lethality. Larvae that survive to the wandering third instar stage often undergo premature histolysis. At a qualitative level, the neuromuscular junctions of the heat shocked larvae are structurally disordered, with reduced branches and smaller, abnormally shaped boutons. Muscle 4 terminals show no significant alterations in the number of synaptic branches or type I boutons. In contrast, muscle 12 terminals show a significant decrease in the number of total synaptic branches, resulting primarily from a loss of higher order branches, and a highly significant decrease in the number of type I boutons.

(Beumer et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mys^hs.PB is a suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

mys^hs.PB is a suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

mys^hs.PB is a suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

mys^hs.PB is a suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Suppression of wing vein phenotypes due to sog^EP7 or sog^EP11 by mys^hs.PB, is strongest when heat shock is administered 20-28 hours after puparium formation.

(Araujo et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

mys^hs.PB rescues mys^{olfC-A459.1F1}

(Ayyub et al., 2000)

mys^hs.PB rescues mys^olfC-x3

(Ayyub et al., 2000)

mys^hs.PB rescues mys^olfC-x17

(Ayyub et al., 2000)

mys^hs.PB rescues mys⁸

(Longley and Ready, 1995)

mys^hs.PB rescues mys⁸

(Brabant and Brower, 1993)

mys^hs.PB rescues mys^ts3

(Bunch et al., 1992)

Comments

Rescues retinal floor defects of mys⁸ mutants.

(Longley and Ready, 1995)

Heat shock induced expression rescues the wing blister phenotype of mys⁸ if³ double mutants. Maximal rescue requires heat shock initiating 5.5 or more days prior to eclosion and continuing up to 1.5 days prior to eclosion.

(Brabant and Brower, 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transfected into S2 cells.

(Curtin et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mys^hs.PB

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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