FlyBase Allele Report: Dmel\osk[bcd.UTR]

General Information

Symbol

Dmel\osk^bcd.UTR

Species

D. melanogaster

Name

FlyBase ID

FBal0035318

Feature type

allele

Associated gene

Dmel\osk

Associated Insertion(s)

Carried in Construct

P{osk-bcd3'UTR}

Also Known As

osk-bcd3'UTR, osk-bcd 3'UTR, osk-bcd, osk-bcd3''UTR

Key Links

Transgenic product class

wild_type

(Ephrussi and Lehmann, 1992)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ephrussi and Lehmann, 1992)

Mutagen

in vitro construct

(Ephrussi and Lehmann, 1992)

Progenitor genotype

Carried in construct

P{osk-bcd3'UTR}

Cytology

Description

Construct: osk promoter sequences from about -1750 from the transcription start site drive expression of osk, in which the osk 3' untranslated region (3'UTR) is replaced by bcd 3'UTR sequences. The nos response elements are lacking in the bcd 3'UTR sequences.

(Ephrussi and Lehmann, 1992)

Allele components

Component

Use(s)

Encoded product / tool

osk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | dominant

(Ephrussi and Lehmann, 1992)

lethal | maternal effect

(Szuperak et al., 2005)

Phenotype Manifest In

abdomen

(Erdelyi et al., 1995)

embryo | anterior

(Anne et al., 2007)

primordial germ cell | ectopic

(Suyama et al., 2009, Anne and Mechler, 2005)

primordial germ cell | maternal effect | increased number

(Megosh et al., 2006)

Detailed Description

Statement

Reference

osk^bcd.UTR causes formation of ectopic pole cells at the anterior in 43% of embryos.

(Suyama et al., 2009)

osk^bcd.UTR embryos have a bicaudal phenotype.

(Anne et al., 2007)

Embryos from mothers that carry 4 copies of the osk^bcd.UTR transgene show approximately double the number of pole cells compared to wild-type embryos.

(Megosh et al., 2006)

Expression of osk^bcd.UTR results in ectopic anterior pole cell formation in embryos.

(Anne and Mechler, 2005)

P{osk-bcd3'UTR} and P{osk-bcd3'UTR}; Tm1^gs1/Tm1^gs1 embryos form two abdomens of opposite polarity.

(Erdelyi et al., 1995)

Homozygous embryos develop mirror image duplication of posterior abdomen and telson structures. In early embryos pole cells are formed at the anterior and posterior poles.

(Ephrussi and Lehmann, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

osk^bcd.UTR has primordial germ cell | ectopic phenotype, suppressible by Lasp^y41

(Suyama et al., 2009)

osk^bcd.UTR has embryo | anterior phenotype, suppressible by csul^P

(Anne et al., 2007)

osk^bcd.UTR has primordial germ cell | ectopic phenotype, suppressible by vls³

(Anne and Mechler, 2005)

NOT Suppressor of

Statement

Reference

osk^bcd.UTR is a non-suppressor of primordial germ cell phenotype of csul^P

(Anne et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of osk^bcd.UTR in a Lasp^y41 mutant background results in only 10% of blastoderm embryos showing pole cell formation at the anterior. All fertilised eggs develop into embryos that display the bicaudal phenotype.

(Suyama et al., 2009)

csul^P suppresses the bicaudal phenotype of osk^bcd.UTR embryos. The embryos produced have cuticle with a normal polarity, but the head is malformed. Pole cells are not formed in these double mutant embryos.

(Anne et al., 2007)

The formation of ectopic anterior pole cells seen in osk^bcd.UTR embryos is suppressed in vls³ blastoderm embryos.

(Anne and Mechler, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

osk^3'-UTR-bcd

(Bratu et al., 2003)

osk^bcd.UTR

Name Synonyms

Secondary FlyBase IDs

References (35)

Report Sections

Open Close