FlyBase Allele Report: Dmel\Lasp[y41]

General Information

Symbol

Dmel\Lasp^y41

Species

D. melanogaster

Name

FlyBase ID

FBal0243457

Feature type

allele

Associated gene

Dmel\Lasp

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

hobo activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Suyama et al., 2009)

Mutagen

hobo activity

(Suyama et al., 2009)

Progenitor genotype

P{wHy}Lasp^DG14505

(Suyama et al., 2009)

Cytology

Description

Imprecise excision of the P{wHy}Lasp^DG14505 resulting in a 3514 bp deletion (with respect to the original insertion point of P{wHy}Lasp^DG14505).

(Suyama et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:16,678,928..16,682,441 [-]

Comment:

Reported as a deletion of 3514 bp from the site of the P{wHy}Lasp^DG14505 insertion at nt 16675541 (release 5.7).

(Suyama et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal hatching behavior

(Suyama et al., 2009)

partially lethal - majority die

(Suyama et al., 2009)

Phenotype Manifest In

primordial germ cell | ectopic

(Suyama et al., 2009)

sarcomere

(Fernandes and Schöck, 2014)

sarcomere (with Lasp¹)

(Fernandes and Schöck, 2014)

striated muscle thin filament

(Fernandes and Schöck, 2014)

striated muscle thin filament (with Lasp¹)

(Fernandes and Schöck, 2014)

Detailed Description

Statement

Reference

Indirect flight muscles from Lasp^y41 and Lasp¹/Lasp^y41 mutants show no gross defects, but sarcomeres as well as thin filaments are 12% shorter than wild type.

(Fernandes and Schöck, 2014)

40-50% of Lasp^y41 mutant eggs fail to develop due to lack of fertilisation. The remaining embryos hatch and develop normally. Homozygous mutant Lasp^y41 females all contain normally developed ovaries.

20% of embryos of Lasp^y41/+ females develop pole cells at the anterior end of the embryo.

(Suyama et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype, suppressible by Scer\GAL4^oc.1.6/Lasp^L.UASp

(Suyama et al., 2009)

NOT suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype, non-suppressible by Scer\GAL4^oc.1.6/Lasp^WA.UASp

(Suyama et al., 2009)

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype, non-suppressible by Lasp^S.UASp/Scer\GAL4^oc.1.6

(Suyama et al., 2009)

Other

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype

(Suyama et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype, suppressible by Scer\GAL4^oc.1.6/Lasp^L.UASp

(Suyama et al., 2009)

NOT suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype, non-suppressible by Scer\GAL4^oc.1.6/Lasp^WA.UASp

(Suyama et al., 2009)

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype, non-suppressible by Lasp^S.UASp/Scer\GAL4^oc.1.6

(Suyama et al., 2009)

Suppressor of

Statement

Reference

Lasp^y41 is a suppressor of embryonic head phenotype of Scer\GAL4^{VP16.mat.αTub67C}, osk^{M1R.fs(1)K10.3'UTR.UAS}

(Suyama et al., 2009)

Lasp^y41 is a suppressor of embryonic thorax phenotype of Scer\GAL4^{VP16.mat.αTub67C}, osk^{M1R.fs(1)K10.3'UTR.UAS}

(Suyama et al., 2009)

Lasp^y41 is a suppressor of primordial germ cell | ectopic phenotype of osk^bcd.UTR

(Suyama et al., 2009)

Other

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype

(Suyama et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Lasp^y45, osk¹/Lasp^y41 females have a grandchildless phenotype, which is rescued by expression of Lasp^{L.Scer\UAS.P\T} under the control of Scer\GAL4^otd. The number of pole cells is partially rescued, increasing to 25 pole cells in stage 11 embryos.

The grandchildless phenotype of Lasp^y45, osk¹/Lasp^y41 females is not rescued by expression of Lasp^{WA.Scer\UAS.P\T} or Lasp^{S.Scer\UAS.P\T} under the control of Scer\GAL4^otd. There is no increase in the number of pole cells observed in these embryos.

The anterior patterning defects observed in embryos expressing osk^{M1R.fs(1)K10.3'UTR.Scer\UAS} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} are significantly reduced in penetrance and severity in a Lasp^y41 background.

(Suyama et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Lasp^y41 is rescued by Scer\GAL4^{VP16.mat.αTub67C}/Lasp^L.UASp

(Suyama et al., 2009)

Comments

Expression of Lasp^{L.Scer\UAS.P\T} restores the hatching defect in Lasp^y41 mutants.

(Suyama et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lasp^y41

(Fernandes and Schöck, 2014)

lasp^y41

(Suyama et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

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