FlyBase Allele Report: Dmel\pb[hs.PP]

General Information

Symbol

Dmel\pb^hs.PP

Species

D. melanogaster

Name

heat shock construct of Percival-Smith

FlyBase ID

FBal0035351

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

P{hs-pb.P}

Key Links

Transgenic product class

wild_type

(Percival-Smith et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Percival-Smith et al., 1997)

Mutagen

in vitro construct

(Percival-Smith et al., 1997, Percival-Smith and Gilfoyle, 1995)

Progenitor genotype

Carried in construct

P{hs-pb.P}

Cytology

Description

Full length pb gene is expressed from an Hsp70 promoter.

(Percival-Smith et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Percival-Smith et al., 1997)

visible

(Percival-Smith et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of pb^hs.PP using a 50 minute heat shock at 5.5 hours of development results in inhibition of germband retraction and head involution. Reduction of the T1 beard to less than or equal to 5 rows of denticles occurs in 95% of first instar larvae.

(Percival-Smith and Bondy, 1999)

One 10-20 minute heat shock at 5 hours AEL results in inhibition of germ band retraction, inhibition of head involution and reduction of the T1 beard. Heat induced expression during third instar larval stage by administration of a 10 minute heat shock is very toxic. Survivors exhibit a number of leg phenotypes including tarsus-to-arista transformation at the ends of all tarsi. Ectopic expression of Scr^hs.PP results in the formation of ectopic T1 beards in T2 and T3, coexpression with pb^hs.PP suppresses T1 beard formation in all three thoracic segments. Ectopic coexpression of Scr^hs.PP and pb^hs.PP at 3 hours AEL does not affect the ectopic salivary gland phenotype caused by ectopic Scr expression alone.

(Percival-Smith et al., 1997)

Heat shock to embryos results in inhibition of germ-band retraction, inhibition of head involution, and a reduction of the T1 beard. One short heat shock of 15-30 mins between 64-72 hrs after egg laying causes shortening of the first leg, increase in the number of sex combs and induction of extra sex combs on the lower tarsal segment of the first leg.

(Percival-Smith and Gilfoyle, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

pb^hs.PP is a suppressor of T1 beard | ectopic phenotype of Scr^hs.PP

(Percival-Smith and Bondy, 1999)

pb^hs.PP is a suppressor of larval mesothoracic segment phenotype of Scr^hs.PP

(Percival-Smith and Bondy, 1999)

pb^hs.PP is a suppressor of larval metathoracic segment phenotype of Scr^hs.PP

(Percival-Smith and Bondy, 1999)

NOT Suppressor of

Statement

Reference

pb^hs.PP is a non-suppressor of T1 beard | ectopic phenotype of Mmus\Hoxa5^hs.PZ

(Percival-Smith and Bondy, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

pb^hs.PP suppresses (down to 4%) the ectopic T1 beard in thoracic segment T2 and/or T3 phenotype seen in 72% of Scr^hs.PP first instar larvae treated with a 50 minute heat shock at 5.5 hours of development.

(Percival-Smith and Bondy, 1999)

Xenogenetic Interactions

Statement

Reference

pb^hs.PP does not suppress the ectopic T1 beard in thoracic segment T2 and/or T3 phenotype seen in 98% of Mmus\Hoxa5^hs.PZ first instar larvae treated with a 50 minute heat shock at 5.5 hours of development.

(Percival-Smith and Bondy, 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pb^hs.PP

Name Synonyms

heat shock construct of Percival-Smith

(Percival-Smith et al., 1997, Percival-Smith and Gilfoyle, 1995)

Secondary FlyBase IDs

References (3)

Report Sections

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