Aversive memory after associative linalool/quinine hemisulfate conditioning is normal in larvae expressing CrebB-17A^b.hs using heat shock.

CrebB-17A^b.hs disrupts the change in sleep need in socially enriched adults after exposure to social impoverishment.

CrebB-17A^b.hs mutants are impaired in 24 hour memory regardless of heat-shock or not.

Long-term memory is impaired in CrebB-17A^b.hs mutants, with or without heat-shock.

Induction of CrebB-17A^b.hs has no effect at time 0 minutes on memory aquisition and/or short term memory. In contrast, memory retention is significantly impaired by the induction of CrebB-17A^b.hs; memory trace in heat-shock-induced larvae becomes undetectable by 60 mins. Induction of CrebB-17A^b.hs suppresses a large part of 30 minute memory.

The olfactory and gustatory responses of CrebB-17A^b.hs larvae are normal compared with wild-type and not altered by heat-shock treatment.

Reduction in 24 hour memory of non-heat-shocked flies relative to Canton S. In addition LTM is significantly reduced with heat shock.

Like wild-type males, non-heat-shocked CrebB-17A^b.hs males that have undergone courtship conditioning (kept in the presence of a female for 7 hours) spend significantly less time engaged in courtship behaviour when placed with a female 5 days after conditioning than males of the same genotype. This significant difference is abolished if these flies are heat-shocked 3 hours before conditioning (a treatment which does not affect courtship behaviour during conditioning), but not if they are heat shocked after conditioning. Identical heat shock treatments to wild-type males do not have this effect. However, when courtship conditioning is reduced to 30 minutes, the presence of CrebB-17A^b.hs plus pre-conditioning heat shock does not eliminate reduction in courtship behaviour 1 hour after conditioning.

The courtship frequency of heat shocked CrebB-17A^b.hs females mated to wild-type males is higher than in control matings during a 1 minute period. The 3-minute courtship frequency of heat shocked CrebB-17A^b.hs females mated to wild-type males is not significantly different from control matings, suggesting that females overexpressing CrebB-17A^b.hs attract males for courtship more quickly than control females. The mean courtship latency of wild-type males mated to heat shocked CrebB-17A^b.hs females is shorter than in control matings. The mating frequency of heat shocked CrebB-17A^b.hs females mated to wild-type males is higher than in control matings during a 10 minute period. The 30-minute mating frequency of heat shocked CrebB-17A^b.hs females mated to wild-type males is not significantly different from control matings, suggesting that females overexpressing CrebB-17A^b.hs start to mate more quickly than control females. The mean courtship duration is lower in heat shocked CrebB-17A^b.hs females mated to wild-type males than in control matings. The courtship index of wild-type males mated to heat shocked CrebB-17A^b.hs females is not significantly different from the courtship index of wild-type males mated to control females. The percentage of heat shocked CrebB-17A^b.hs females that do not reject a copulation attempt by wild-type males is significantly higher than that of control females. The locomotor activity of females expressing CrebB-17A^b.hs is normal.

Expression of CrebB-17A^b.hs in a wild-type background has no effect on synapse size (bouton number) or strength (EJC amplitude) at the larval neuromuscular junction.

Mutant flies show a significant increase in the number of rest hours per 24 hours compared to controls, even in the absence of heat shock.

Expression of CrebB^b.hs using heat shock significantly increases and prolongs the mean daily rest rebound after rest deprivation.

Heat induced expression in wild type during larval stages primarily alters synaptic function rather than morphology as shown by the excitatory postsynaptic potential (EPSP) of each synaptic junction. Expression of CrebB-17A^b.hs does not alter the increase in dnc^M14 synaptic structure but does reduce the quantal content to wild type levels. Expression also significantly suppresses dnc-mediated depression at stimulation frequencies above 10Hz.

Heat induction disrupts one day memory after spaced training, and long term memory though it has no effect on initial learning, olfactory acuity or shock reactivity. The effect on long term memory is very similar to the effect of cycloheximide treatment on wild type flies. Double mutants with rad¹ show zero one day retention after spaced training.