FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lacZβTub56D.AS3
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General Information
Symbol
Ecol\lacZβTub56D.AS3
Species
E. coli
Name
FlyBase ID
FBal0040501
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{lacZβTub56D.AS3}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Buttgereit, 1993)
Progenitor genotype
Carried in construct
P{lacZβTub56D.AS3}
Cytology
Description

Starting with plasmid pWHL, a 850bp fragment of the βTub56D gene intron was cloned upstream of a truncated heat shock promoter in front of a lacZ reporter gene at a unique StuI site in the multiple cloning site.

(Buttgereit, 1993)
Allele components
Component
Use(s)
Regulatory region(s)
betaTub56D_AS3
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 14
      larval intersegmental apodeme | precursor
      (Buttgereit, 1993)
      embryonic/larval intrasegmental apodeme | precursor
      (Buttgereit, 1993)
      embryonic stage 15 -- 17
      embryonic/larval A1-7 intrasegmental apodeme 1
      (Buttgereit, 1993)
      embryonic/larval A1-7 intrasegmental apodeme 2
      (Buttgereit, 1993)
      embryonic/larval A1-7 intersegmental apodeme
      (Buttgereit, 1993)
      embryonic/larval A1-7 pleural external apodeme
      (Buttgereit, 1993)
      Additional Information
      Statement
      Reference

      EcollacZβTub56D.AS3 reporter gene expression is not observed before embryonic stage 14 and is observed in the intersegmental and intrasegmental apodemes. Expression in the embryonic/larval A1-7 pleural external apodeme ('petapo' or pet apodemes) is stronger than that of EcollacZβTub56D.AS2.

      (Buttgereit, 1993)
       
      Marker for
      Reflects expression of
      βTub56D
      (Buttgereit, 1993)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZβTub56D.AS3
      lacZβTub56D.AS3
      Name Synonyms
      Secondary FlyBase IDs
        References (2)