FB2026_03 , released September 17, 2026
Recombinant Construct: P{lacZβTub56D.AS3}
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General Information
Symbol
P{lacZβTub56D.AS3}
FlyBase ID
FBtp0003925
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZβTub56D.AS3
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZβTub56D.AS3
Transgenic product class
Component
Use(s)
Regulatory region(s)
betaTub56D_AS3
Encoded product / tool
lacZ
Description

Starting with plasmid pWHL, a 850bp fragment of the βTub56D gene intron was cloned upstream of a truncated heat shock promoter in front of a lacZ reporter gene at a unique StuI site in the multiple cloning site.

(Buttgereit, 1993)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14
larval intersegmental apodeme | precursor
(Buttgereit, 1993)
embryonic/larval intrasegmental apodeme | precursor
(Buttgereit, 1993)
embryonic stage 15 -- 17
embryonic/larval A1-7 intrasegmental apodeme 1
(Buttgereit, 1993)
embryonic/larval A1-7 intrasegmental apodeme 2
(Buttgereit, 1993)
embryonic/larval A1-7 intersegmental apodeme
(Buttgereit, 1993)
embryonic/larval A1-7 pleural external apodeme
(Buttgereit, 1993)
Additional Information
Statement
Reference

EcollacZβTub56D.AS3 reporter gene expression is not observed before embryonic stage 14 and is observed in the intersegmental and intrasegmental apodemes. Expression in the embryonic/larval A1-7 pleural external apodeme ('petapo' or pet apodemes) is stronger than that of EcollacZβTub56D.AS2.

(Buttgereit, 1993)
 
Marker for
Reflects expression of
βTub56D
(Buttgereit, 1993)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacZβTub56D.AS3}
(FlyBase, 1996-)
P{w+,lacZβTub56D.AS3}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (4)