enaGC5/enaGC8 embryos show modest levels of midline crossing errors by axons in the central nervous system.
29% of ISNb axons show a bypass phenotype in enaGC5/enaGC8 embryos. A small number of RP neurons follow an aberrant path within the central nervous system. 7% of ISNb axons show a bypass phenotype in enaGC8/+ embryos.
Heterozygotes with enaGC1 have diffuse and loosely bundled longitudinal and commissural axon tracts. Some heterozygotes with enaGC5 also exhibit axon misguiding. Some homozygous embryos exhibit thinning of longitudinal connectives, increased number of axons exciting the CNS from the longitudinal axons or failure of commissural axons to separate into anterior and posterior axon bundles. The overall organisation of the PNS is disrupted, spacing and organisation of the neurons is irregular and some clusters of neurons are mislocalised.