FlyBase Allele Report: Dmel\Poxn[hs.sev]

General Information

Symbol

Dmel\Poxn^hs.sev

Species

D. melanogaster

Name

FlyBase ID

FBal0045005

Feature type

allele

Associated gene

Dmel\Poxn

Associated Insertion(s)

Carried in Construct

P{hsp(sE)-poxn}

Key Links

Transgenic product class

cDNA

(Dambly-Chaudiere et al., 1992)

wild_type

(Dambly-Chaudiere et al., 1992)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Dambly-Chaudiere et al., 1992)

wild_type

(Dambly-Chaudiere et al., 1992)

Mutagen

in vitro construct

(Dambly-Chaudiere et al., 1992)

Progenitor genotype

Carried in construct

P{hsp(sE)-poxn}

Cytology

Description

P4c6 Poxn cDNA expression is driven by an XbaI-KpnI fragment of the first 95bp of Hsp70 leader and 250bp of Hsp70 upstream sequence and a 1.2kb sev enhancer fragment. Transcription is terminated by a 0.8kb tubulin fragment.

(Dambly-Chaudiere et al., 1992)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

Poxn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Vervoort et al., 1995)

visible

(Jiao et al., 2001)

Phenotype Manifest In

chemosensory sensory organ | adult stage | ectopic

(Gautier et al., 1997)

chemosensory sensory organ | ectopic

(del Alamo et al., 2002)

chemosensory sensory organ | ectopic | larval stage

(Gautier et al., 1997)

chordotonal organ

(Vervoort et al., 1995)

cone cell

(Jiao et al., 2001)

external sensory organ

(Vervoort et al., 1995)

eye

(Jiao et al., 2001, Zeidler et al., 1996)

Kolbchen | embryonic stage

(Dambly-Chaudiere et al., 1992)

mechanosensory sensory organ

(del Alamo et al., 2002)

mechanosensory sensory organ & tibia & adult

(Nottebohm et al., 1994)

mechanosensory sensory organ & tibia & neuron & adult

(Nottebohm et al., 1994)

ommatidium

(Jiao et al., 2001)

Detailed Description

Statement

Reference

When Poxn^hs.sev is heatshock induced for 30 minutes at 37^oC at 8 hours APF, mechanosensory bristles are transformed into chemosensory bristles.

(del Alamo et al., 2002)

Flies expressing Poxn^hs.sev have a rough eye phenotype. The arrangement, shape and number of cone cells in mid-pupal eye discs is disturbed.

(Jiao et al., 2001)

Overexpression causes development of ectopic chemosensory organs in the larva and adult.

(Gautier et al., 1997)

Rough eye phenotype unaffected by TfIIA-S^E73.

(Zeidler et al., 1996)

Expression during embryogenesis leads to the formation of an excess of p-es organs (FBrf0055546) due to transformation of many ch and m-es organs into p-es organs.

(Vervoort et al., 1995)

Induction 10 hours after puparium formation (APF), at the time when the mechanosensory SMCs emerge, causes morphological transformation of mechanosensory bristles into chemosensory bristles. Ultrastructure analysis of the shaft reveals it comprises of two lumens: a tubular compartment with contains two to five dendrites and a crescent-like compartment which contains no dendrites. Inducing expression 14 and 15 hours APF results in transformation of the external morphology of some mechanosensory bristles. No transformation is observed when expression is induced 16 hours APF or later. DiI labelling of sensory neurons at 16 and 20 hours APF shows a few cases of mechanosensory bristles innervated by several neurons and more frequently a group of unconnected neurons that do not connect to the external shaft.

(Nottebohm et al., 1994)

Embryos display supernumerary kolbchen in all thoracic segments.

(Dambly-Chaudiere et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Poxn^hs.sev has visible phenotype, suppressible by sv^sev.PJ

(Jiao et al., 2001)

Poxn^hs.sev has visible phenotype, suppressible by sv^+mFa

(Jiao et al., 2001)

Enhancer of

Statement

Reference

Poxn^hs.sev is an enhancer of visible phenotype of sv^spa-pol

(Jiao et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Poxn^hs.sev has eye phenotype, suppressible by sv^sev.PJ

(Jiao et al., 2001)

Poxn^hs.sev has eye phenotype, suppressible by sv^+mFa

(Jiao et al., 2001)

Enhancer of

Statement

Reference

Poxn^hs.sev is an enhancer of cone cell phenotype of sv^spa-pol

(Jiao et al., 2001)

Poxn^hs.sev is an enhancer of eye phenotype of sv^spa-pol

(Jiao et al., 2001)

Other

Statement

Reference

Poxn^hs.sev, sv^spa-pol has lens phenotype

(Jiao et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

When animals containing Poxn^hs.sev and phl^F20.Scer\UAS (driven by Scer\GAL4^hs.PB) and heatshocked, an additive phenotype is seen, mechanosensory bristles are transformed into chemosensory bristles and all epidermal cells are transformed into bracts. A similar effect is seen by the combination of Poxn^hs.sev and Poxn^hs.sev (driven by Scer\GAL4^hs.PB).

(del Alamo et al., 2002)

The sv^spa-pol eye phenotype is enhanced by Poxn^hs.sev, with the eye lacking all lenses and most bristles. The number of cone cells present in mid-pupal eye discs is reduced compared to sv^spa-pol single mutants and those that are seen are smaller than normal and seem to be undergoing apoptosis. One copy of sv^sev.PJ or sv^+mFa rescues the Poxn^hs.sev eye phenotype to wild type.

(Jiao et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pox-n^hs.sev

(Nottebohm et al., 1994)

Poxn^hs.sev

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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