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General Information
Symbol
Dmel\enUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Guillen
FlyBase ID
FBal0045599
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-en, UAS.en
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a 2kb EcoRI fragment including cDNA of the entire en ORF is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

embryonic/larval pericardial cell & embryonic myoblast, with Scer\GAL4how-24B, Scer\GAL4twi.PG

fat body/gonad primordium & parasegment 4, with Scer\GAL4twi.PGa

fat body/gonad primordium & parasegment 5, with Scer\GAL4twi.PGa

fat body/gonad primordium & parasegment 6, with Scer\GAL4twi.PGa

fat body/gonad primordium & parasegment 6, with Scer\GAL4zen.Kr.PF

fat body/gonad primordium & parasegment 7, with Scer\GAL4twi.PGa

fat body/gonad primordium & parasegment 7, with Scer\GAL4zen.Kr.PF

fat body/gonad primordium & parasegment 8, with Scer\GAL4twi.PGa

fat body/gonad primordium & parasegment 8, with Scer\GAL4zen.Kr.PF

fat body/gonad primordium & parasegment 9, with Scer\GAL4twi.PGa

Detailed Description
Statement
Reference

Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing enScer\UAS.cGa under the control of Scer\GAL4sim.P3.7.

Expression of enScer\UAS.cGa under the control of Scer\GAL4T155 results in transformation of anterior compartment to posterior compartment structures in the tergites.

When expression is driven by Scer\GAL4twi.PG and Scer\GAL4how-24B pericardial and muscle progenitors are missing and only a few mesodermal cells remain. Visceral mesodermal cells and fat body are unaffected.

When expression is driven by Scer\GAL4Ubx.PdC in clones in the Posterior compartment of the developing adult abdominal tergite there is generally little effect, with some exceptions. The abnormal clones are elongated and abut the A/P border for many cell diameters from the posterior side. They make a6/p3 cuticle, with irregular hairs. These clones originate in the A compartment and merge later into the P compartment. When expression is driven by Scer\GAL4Ubx.PdC in clones in the Anterior compartment of the developing adult abdominal tergite, clones are large and have reversed polarity (and induce reversed polarity) with an ectopic P region. New A/P and P/A borders are formed at the anterior and posterior limits of the clone. Marked bristles are rare, suggesting a posterior identity. In the most posterior parts of the A compartment larger clones are rare, and, when expression is driven by Scer\GAL469B, have p3 identity (with hairs). Hairs in front of the clone are oriented normally, behind the clone they are reversed. Clones induced in pupal stages are small and p1/p2 identity (in the anterior domain) or a6/p3, with varying polarities (in the posterior domain). The type of cuticle is characteristic of the segment in which it is found.

Scer\GAL4twi.PGa-mediated overexpression of en leads to an expansion of the primary clusters resulting in a continuous dorsolateral fat body primordium in parasegments 4-9. Expression also causes expansion of the visceral mesoderm primordia and somatic gonad primordia. Primordia of the somatic muscles and heart are abolished. At stage 11 Scer\GAL4zen.Kr.PF-mediated expression expands the dorsolateral fat body primordium in parasegments 6-8, the secondary central fat body clusters are missing in parasegments 6-8.

Scer\GAL4arm.PS mediated expression causes small and spherical embryos, unsegmented with a central stripe of weak denticles of row 1 type.

When expression is driven by Scer\GAL4Bx-MS1096, anterior dorsal wing develops a posterior pattern. Double row hairs and the axillary cord appear in the anterior compartment. When expression is driven by Scer\GAL471B or Scer\GAL4C-734, vein pattern of part of the anterior compartment is transformed into posterior pattern. When expression is driven by Scer\GAL430A, anterior wing patterns are duplicated, the costa is transformed into axillary cord, along with a duplication of adjacent anterior patterns. Scer\GAL4Bx-MS1096 can also generate anterior pattern in posterior positions, such that posterior compartments resemble those of en1/en1 or en1/en2.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

enScer\UAS.cGa; Scer\GAL4prd.RG1 rescues metameric furrow formation in Df(2R)enE; wgl-17 double homozygous embryos, but these furrows disappear prematurely (before stage 13) on the ventral side of the embryo. Metameric furrow formation is also rescued in Df(2R)enE homozygous embryos by enScer\UAS.cGa; Scer\GAL4btd-MD808, but in this case the furrows persist ventrally into stage 14.

Scer\GAL4arm.PS mediated expression of both wgScer\UAS.cLa and enScer\UAS.cGa in wgl-17 Df(2R)enE embryos causes a small beardless, near-spherical and unsegmented embryo with extruded organs (believed to be foregut and hindgut), i.e. less phenotypic rescue than for Scer\GAL4arm.PS mediated expression of wgScer\UAS.cLa alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
enScer\UAS.cGa
enUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Guillen
Secondary FlyBase IDs
    References (17)