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General Information
Symbol
Dmel\scrak08255
Species
D. melanogaster
Name
FlyBase ID
FBal0046055
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion within the 5'UTR, 176bp from the translational start codon.

Insertion components
P{lacW}scrak08255
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The lateral chordotonal 5 (Lch5) neurons of scrak08255/scra8 embryos are shifted dorsally, disorganised and enlarged compared to wild type. There is an increase in the amount of DNA within the enlarged Lch5 neurons, indicative of multinucleate cells. There is a loss of Lch5 ligament cells in the mutant embryos, with the mutant embryos having an average of two ligament cells per Lch5 cluster (compared to five in wild-type embryos). The cap cells are present in normal number and orientation in the mutant Lch5 clusters.

scrak08255/scra8 embryos show a drastic decrease in the number of scolopale/sheath cells throughout the peripheral nervous system. The lack of scolopale cells is particularly evident in the Ch lineages, with the five neurons of the Lch5 clusters often having no associated scolopale. No sheath cells are detectable in the ventral' abdominal compartment of the mutant embryos (three sheath cells are seen in this region in wild-type embryos). The number of external sensory (ES) neurons in the ventral' region is reduced compared to wild type. ES organs of the lateral region tend not to lose sheath cells.

The ventral multidendritic neurons are unchanged in number in scrak08255/scra8 embryos, but they are increased in size. There is no reduction in the number of ventral ES structural cells.

scrak08255/scra8 embryos show no difference in the levels of apoptosis compared to controls from stage 10 until the end of embryogenesis.

scrak08255/scra8 embryos have relatively higher numbers of mitotic cells than wild-type embryos by the end of stage 12. This phenotype becomes more striking during the latter stages of embryonic development. Mitotic bodies are seen within differentiated peripheral nervous system neurons.

Homozygous embryos exhibit an increase in neuronal body size and a concomitant reduction in the total number of neurons due to cell fusion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Lethality not revertible with excision of the P{lacW} insert.

Excision of the insertion can revert the lethal phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
l(2)k0825582-55
l(2)k08255k08255
Name Synonyms
Secondary FlyBase IDs
    References (4)