FlyBase Allele Report: Dmel\l(3)mbt[E2]

General Information

Symbol

Dmel\l(3)mbt^E2

Species

D. melanogaster

Name

FlyBase ID

FBal0046090

Feature type

allele

Associated gene

Dmel\l(3)mbt

Associated Insertion(s)

Carried in Construct

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wismar et al., 1995, Gateff and Mechler, 1989)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3R:27,267,641..27,267,641 [-]

Nucleotide change:

G27267641A

Amino acid change:

W1099term | l(3)mbt-PB

Reported amino acid change:

W1099term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Yohn et al., 2003)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: W1099term.

(Yohn et al., 2003)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal (with l(3)mbt^3d23)

(Ambrus et al., 2009)

lethal (with l(3)mbt^3d33)

(Ambrus et al., 2009)

lethal (with l(3)mbt^4d16)

(Ambrus et al., 2009)

lethal (with l(3)mbt^4d30)

(Ambrus et al., 2009)

lethal (with l(3)mbt^5d6)

(Ambrus et al., 2009)

lethal (with l(3)mbt^5d8)

(Ambrus et al., 2009)

lethal (with l(3)mbt^6a14)

(Ambrus et al., 2009)

lethal (with l(3)mbt^6a31)

(Ambrus et al., 2009)

mitotic cell cycle defective | embryonic stage | maternal effect (with Df(3R)mbt^PE3)

(Yohn et al., 2003)

tumorigenic | recessive

(Gateff and Mechler, 1989)

Phenotype Manifest In

cleavage nucleus | maternal effect (with Df(3R)mbt^PE3)

(Yohn et al., 2003)

embryonic/larval brain

(Gateff and Mechler, 1989)

Detailed Description

Statement

Reference

In cleavage stage embryos laid by l(3)mbt^E2/Df(3R)mbt^PE3 mothers the nuclei stop dividing leaving most embryos with only 2-16 nuclei.

(Yohn et al., 2003)

Homozygous larvae develop brain tumours.

(Gateff and Mechler, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

l(3)mbt^E2/l(3)mbt[+] is a non-enhancer of visible phenotype of CycE^JP

(Brumby et al., 2004)

NOT Suppressor of

Statement

Reference

l(3)mbt^E2/l(3)mbt[+] is a non-suppressor of visible phenotype of CycE^JP

(Brumby et al., 2004)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

l(3)mbt^E2/l(3)mbt[+] is a non-enhancer of eye phenotype of CycE^JP

(Brumby et al., 2004)

NOT Suppressor of

Statement

Reference

l(3)mbt^E2/l(3)mbt[+] is a non-suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

In trans Df(3R)D605 or Df(3R)mbt^PE3, l(3)mbt alleles form the following phenotypic series: l(3)mbt^GM161 < l(3)mbt^ts1 < l(3)mbt^GM79 < l(3)mbt^E2 < l(3)mbt^GM76. Phenotypes range from weak maternal-effect mitotic asynchrony to a severe mitotic block to oogenesis defects in trans-heterozygous females. Each of these phenotypes is temperature sensitive: phenotypes increase in severity as the temperature is raised from 18 to 29^oC.

(Yohn et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

l(3)mbt^E2

(Richter et al., 2011, Janic et al., 2010, Ambrus et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close