56.7% of fertilised eggs derived from polo¹/polo^DD6 females have two major bipolar anastral spindles 30 minutes after oviposition, indicating arrest in meiosis. 15.3% have 3 spindles and 11.3% have 4 spindles, suggesting some progression through anaphase/telophase of the second meiosis. In these cases, chromosomes are found at the equator of spindles or scattered within them. No more than 5 anastral spindles are seen in these embryos. The sperm aster splits in two during late metaphase II, but its centrosome is unable to drive the formation of a biastral spindle, so that the male haploid complement is associated with a bipolar anastral spindle.

Transheterozygotes are female sterile, exhibit abdominal, brain and meiotic defects. Postmeiotic onion stage cysts are abnormal with large variation in nuclear sizes reflecting both mitotic and meiotic nondisjunction.