In flies transformed withSry-alphaFBtr0091350:pb-XREcollacZ constructs, EcollacZ expression was detected in thecellular blastoderm embryo in all but the pole cells. Expression wasweaker in the Ecol\lacZSry-alpha.CalphaNbetaG and theEcol\lacZSry-alpha.CalphaN'betaG lines, which lack Sry-alpha sequencesupstream from -311. The same two lines also showed neural expressionduring embryogenesis, which was not seen with the other lines, or in wildtype Sry-alpha. The -118 to +130 region was sufficient for cellularblastoderm expression, which was enhanced with the -311 to -118 region.The latter domain might contain negative regulatory elements, the removalof which would allow nervous system expression.
