FB2026_03 , released September 17, 2026
Recombinant Construct: P{CαN'βG}
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General Information
Symbol
P{CαN'βG}
FlyBase ID
FBtp0005472
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Component allele Ecol\lacZSry-α.CαN'βG
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Contains Sry-α sequences from -118bp to +130bp in reverse orientation, upstream of a lacZ reporter. Also contains the Sry-δ polyadenylation signal downstream of the lacZ coding sequence.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In flies transformed withSry-alphaFBtr0091350:pb-XREcollacZ constructs, EcollacZ expression was detected in thecellular blastoderm embryo in all but the pole cells. Expression wasweaker in the Ecol\lacZSry-alpha.CalphaNbetaG and theEcol\lacZSry-alpha.CalphaN'betaG lines, which lack Sry-alpha sequencesupstream from -311. The same two lines also showed neural expressionduring embryogenesis, which was not seen with the other lines, or in wildtype Sry-alpha. The -118 to +130 region was sufficient for cellularblastoderm expression, which was enhanced with the -311 to -118 region.The latter domain might contain negative regulatory elements, the removalof which would allow nervous system expression.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{CαN'βG}
Secondary FlyBase IDs
    References (3)