FlyBase Allele Report: Dmel\Lar[UAS.cKa]

General Information

Symbol

Dmel\Lar^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Krueger

FlyBase ID

FBal0048853

Feature type

allele

Associated gene

Dmel\Lar

Associated Insertion(s)

Carried in Construct

P{UAS-Lar.K}

Also Known As

UAS-LAR, UAS-Dlar

Key Links

Transgenic product class

cDNA

(Krueger et al., 1996)

wild_type

(Krueger et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Krueger et al., 1996)

wild_type

(Krueger et al., 1996)

Mutagen

in vitro construct

(Krueger et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-Lar.K}

Cytology

Description

Lar cDNA expression is governed by UAS regulatory sequences.

(Krueger et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Lar

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult s-LNv neuron | adult stage, with Scer\GAL4^Pdf.PU

(Oliva et al., 2016)

axon | adult stage, with Scer\GAL4^Pdf.PU

(Oliva et al., 2016)

bouton | increased number, with Scer\GAL4^elav.PLu

(Kaufmann et al., 2002)

bouton | increased number, with Scer\GAL4^elav-C155

(Kaufmann et al., 2002)

larval abdominal segmental nerve, with Scer\GAL4^elav-C155

(Fox and Zinn, 2005)

neuromuscular junction, with Scer\GAL4^elav.PLu

(Kaufmann et al., 2002)

neuromuscular junction, with Scer\GAL4^elav-C155

(Kaufmann et al., 2002)

NMJ bouton | larval stage, with Scer\GAL4^how-24B, Scer\GAL4^sca.PU

(Johnson et al., 2006)

Detailed Description

Statement

Reference

The expression of Lar^Scer\UAS.cKa under the control of Scer\GAL4^Pdf.PU results in adult sLNv neurons exhibiting significantly longer axons compared to controls.

(Oliva et al., 2016)

Expression of Lar^Scer\UAS.cKa under the simultaneous control of both Scer\GAL4^sca.PU and Scer\GAL4^how-24B results in an increase in bouton number at the neuromuscular junction compared to wild type.

(Johnson et al., 2006)

Expression of Lar^Scer\UAS.cKa, under the control of Scer\GAL4^elav-C155, causes bifurcation of the anterior and posterior branches of the SNa with a frequency of 23%. No ISNb phenotypes are observed.

(Fox and Zinn, 2005)

Expression of Lar^Scer\UAS.cKa during mid-larval development under the control of Scer\GAL4^elav.PLu or Scer\GAL4^elav-C155 results in an increase in bouton number at the muscle 6/7 neuromuscular junction compared to controls.

(Kaufmann et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Lar^UAS.cKa is a non-suppressor of abnormal neuroanatomy | somatic clone | pupal stage phenotype of CadN^Δ14

(Prakash et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lar^UAS.cKa, Scer\GAL4^elav.PLu has bouton | increased number phenotype, suppressible by Liprin-α^1J-0/Liprin-α^R117

(Kaufmann et al., 2002)

Abl^UAS.cFa, Lar^UAS.cKa has larval intersegmental nerve phenotype, suppressible | partially by Scer\GAL4^elav.PLu

(Wills et al., 1999)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Lar^UAS.cKa is a non-suppressor of photoreceptor neuron | somatic clone | pupal stage phenotype of CadN^Δ14

(Prakash et al., 2009)

Lar^UAS.cKa/Scer\GAL4^elav.PLu is a non-suppressor of bouton phenotype of Liprin-α^1J-0/Liprin-α^R117

(Kaufmann et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 in CadN^Δ14 somatic mutant clones does not rescue the photoreceptor targeting defects.

(Prakash et al., 2009)

Liprin-α^1J-0/Liprin-α^R117 larvae expressing Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav.PLu show a reduction in bouton number at the muscle 6/7 neuromuscular junction equivalent to that seen in Liprin-α^1J-0/Liprin-α^R117 single mutants.

(Kaufmann et al., 2002)

Partially suppresses the ISNb bypass phenotype of embryos expressing Abl^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu.

(Wills et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/Lar^UAS.cKa rescues Lar²¹²⁷

(Prakash et al., 2009)

Scer\GAL4^elav.PU/Lar^UAS.cKa rescues Lar^5.5/Lar^13.2

(Johnson et al., 2006)

Scer\GAL4^T155/Lar^UAS.cKa rescues Lar^5.5/Lar^13.2

(Krueger et al., 2003)

Scer\GAL4^T155/Lar^UAS.cKa rescues Lar^5.5/Lar^13.2

(Bateman et al., 2001)

Lar^UAS.cKa/Scer\GAL4^hs.2sev rescues Lar^5.5/Lar²¹²⁷

(Maurel-Zaffran et al., 2001)

Scer\GAL4^elav-C155/Lar^UAS.cKa rescues Lar^unspecified

(Krueger et al., 1996)

Partially rescues

Scer\GAL4^elav-C155/Lar^UAS.cKa partially rescues Lar^5.5/Lar^13.2

(Krueger et al., 2003)

Scer\GAL4^elav-C155/Lar^UAS.cKa partially rescues Lar^5.5/Lar^13.1

(Krueger et al., 2003)

Scer\GAL4^elav-C155/Lar^UAS.cKa partially rescues Lar^5.5/Lar^5.2

(Krueger et al., 2003)

Lar^UAS.cKa/Scer\GAL4^sca-109-68 partially rescues Lar^5.5/Lar²¹²⁷

(Maurel-Zaffran et al., 2001)

Fails to rescue

Lar^UAS.cKa/Scer\GAL4^how-24B fails to rescue Lar^5.5/Lar^13.2

(Johnson et al., 2006)

Scer\GAL4^elav-C155/Lar^UAS.cKa fails to rescue Lar^5.5/Lar^13.2

(Krueger et al., 2003)

Lar^UAS.cKa/Scer\GAL4^198Y fails to rescue Lar^5.5/Lar^13.2

(Bateman et al., 2001)

Comments

Expression of Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 almost completely rescues the aberrant axon extension in Lar²¹²⁷ mutant photoreceptor cells.

(Prakash et al., 2009)

Expression of Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU rescues the reduction in bouton number at the neuromuscular junction seen in Lar^5.5/Lar^13.2 larvae, while expression under the control of Scer\GAL4^how-24B does not rescue the mutant phenotype.

(Johnson et al., 2006)

Partial rescue statement based on rescue of lethality. Fails to rescue statement based on larval ISNb and ISNd phenotype. Rescue statement based on rescue of oocyte phenotype of Lar^13.2/Lar^5.5 escapers.

(Krueger et al., 2003)

Rescues the targeting defects of Lar^5.5/Lar²¹²⁷ R7 cell axons when expressed under the control of Scer\GAL4^hs.2sev. Partially rescues the targeting defects of Lar^5.5/Lar²¹²⁷ R7 cell axons when expressed under the control of Scer\GAL4^sca-109-68.

(Maurel-Zaffran et al., 2001)

When expressed from Scer\GAL4^elav-C155, Lar^Scer\UAS.cKa rescues the Lar mutant phenotypes. The SNb choice point defect is almost completely rescued, and the embryonic lethality of Lar^5.5/Lar^13.2 is overcome.

(Krueger et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9149

w^*; P{UAS-Lar.K}P4B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Lar^Scer\UAS.cKa

Lar^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Krueger

(Krueger et al., 1996)

Secondary FlyBase IDs

References (15)

Report Sections

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