FlyBase Allele Report: Dmel\Rbf[GMR.PD]

General Information

Symbol

Dmel\Rbf^GMR.PD

Species

D. melanogaster

Name

glass multimer reporter construct of Du

FlyBase ID

FBal0048929

Feature type

allele

Associated gene

Dmel\Rbf

Associated Insertion(s)

Carried in Construct

P{GMR-Rbf}

Also Known As

GMR-RBF4, GMRRBF^4C

Key Links

Transgenic product class

wild_type

(Du et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Du et al., 1996)

Mutagen

in vitro construct

(Secombe et al., 1998, Du et al., 1996)

Progenitor genotype

Carried in construct

P{GMR-Rbf}

Cytology

Description

GMR regulatory sequences drive expression of a full length Rbf open reading frame.

(Du et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Rbf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage

(Kuzin et al., 2000)

decreased occurrence of cell division | larval stage

(Secombe et al., 1998)

visible

(Duman-Scheel et al., 2002, Kuzin et al., 2000, Secombe et al., 1998, de Nooij et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant animals exhibit fused ommatidia and missing interommatidial bristles.

(Weng et al., 2003)

Flies expressing Rbf^GMR.PD have a rough eye phenotype.

(Duman-Scheel et al., 2002)

Rbf^GMR.PD flies with two copies of the responsible transposon, P{GMR-Rbf}, have no obvious phenotype in the eye. In flies with 4 copies of P{GMR-Rbf}, the posterior of the eye have missing bristles and pigment cells, and some fusion of ommatidia. Heat shock treatment of these flies has no affect on the phenotype. Treatment of Rbf^GMR.PD flies with four copies of P{GMR-Rbf} with the Nos inhibitor L-NAME (which does not affect eye morphology in wild-type flies) almost completely rescues the eye phenotype seen in these flies. The number of cells in S phase also increases.

(Kuzin et al., 2000)

Flies expressing Rbf^GMR.PD have a mild rough eye phenotype. The number of cells entering S phase posterior to the morphogenetic furrow is reduced compared to wild-type.

(Secombe et al., 1998)

The eyes of adults carrying two copies of Rbf^GMR.PD are normal, four copies causes fused ommatidia and lack of bristles.

(Du et al., 1996)

Flies carrying two copies of Rbf^GMR.PD transgene appear wild type, normal complement of retinal cells.

(de Nooij et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rbf^GMR.PD has decreased occurrence of cell division | larval stage phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Rbf^GMR.PD has visible | larval stage phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Suppressed by

Statement

Reference

Rbf^GMR.PD, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^GMR.PD has visible phenotype, suppressible by ptc^su53/ptc[+]

(Duman-Scheel et al., 2002)

Dp^GMR.PD, E2f1^GMR.PD, Rbf^GMR.PD has visible phenotype, suppressible by BacA\p35^GMR.PH

(Kuzin et al., 2000)

Enhancer of

Statement

Reference

Rbf^GMR.PD is an enhancer of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

Rbf^GMR.PD is an enhancer of decreased occurrence of cell division | larval stage phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

Rbf^GMR.PD is a non-enhancer of visible phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

NOT Suppressor of

Statement

Reference

Rbf^GMR.PD is a non-suppressor of visible phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

Rbf^GMR.PD is a non-suppressor of abnormal size | adult stage phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

Other

Statement

Reference

Rbf^GMR.PD, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype

(Weng et al., 2003)

Nos^hs.PK, Rbf^GMR.PD has decreased cell number | adult stage phenotype

(Kuzin et al., 2000)

Nos^GMR.PK, Rbf^GMR.PD has visible phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has visible phenotype

(Kuzin et al., 2000)

Rbf^GMR.PD, dap^GMR.PdN has visible phenotype

(de Nooij et al., 1996)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbf^GMR.PD has ommatidium phenotype, enhanceable by dap^GMR.PdN

(Weng et al., 2003)

Rbf^GMR.PD has interommatidial bristle phenotype, enhanceable by dap^GMR.PdN

(Weng et al., 2003)

Rbf^GMR.PD has phenotype, enhanceable by dap^GMR.PdN

(Xin et al., 2002)

Rbf^GMR.PD has eye disc posterior to the morphogenetic furrow | larval stage phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Rbf^GMR.PD has eye phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Suppressed by

Statement

Reference

Rbf^GMR.PD has interommatidial bristle phenotype, suppressible by Nos^{4.GMR.Tag:FLAG}

(Stasiv et al., 2004)

Rbf^GMR.PD has ommatidium phenotype, suppressible by Nos^{4.GMR.Tag:FLAG}

(Stasiv et al., 2004)

Rbf^GMR.PD has pigment cell phenotype, suppressible by Nos^{4.GMR.Tag:FLAG}

(Stasiv et al., 2004)

Rbf^GMR.PD, dap^GMR.PdN has eye disc & S phase phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^GMR.PD has interommatidial bristle phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^GMR.PD has ommatidium phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^GMR.PD has eye phenotype, suppressible by ptc^su53/ptc[+]

(Duman-Scheel et al., 2002)

Rbf^GMR.PD has phenotype, suppressible by dap^unspecified/dap[+]

(Xin et al., 2002)

Rbf^GMR.PD has phenotype, suppressible by CycD^GMR.PE

(Xin et al., 2002)

Rbf^GMR.PD has eye phenotype, suppressible by BacA\p35^GMR.PH/Dp^GMR.PD/E2f1^GMR.PD

(Kuzin et al., 2000)

Rbf^GMR.PD has ommatidium phenotype, suppressible by BacA\p35^GMR.PH/Dp^GMR.PD/E2f1^GMR.PD

(Kuzin et al., 2000)

Rbf^GMR.PD has pigment cell phenotype, suppressible by BacA\p35^GMR.PH/Dp^GMR.PD/E2f1^GMR.PD

(Kuzin et al., 2000)

Dp^GMR.PD, E2f1^GMR.PD, Rbf^GMR.PD has eye phenotype, suppressible by BacA\p35^GMR.PH

(Kuzin et al., 2000)

Dp^GMR.PD, E2f1^GMR.PD, Rbf^GMR.PD has pigment cell phenotype, suppressible by BacA\p35^GMR.PH

(Kuzin et al., 2000)

Enhancer of

Statement

Reference

Rbf^GMR.PD is an enhancer of eye phenotype of CycE^JP

(Secombe et al., 1998)

Rbf^GMR.PD is an enhancer of eye disc posterior to the morphogenetic furrow | larval stage phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

Rbf^GMR.PD is a non-enhancer of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

Rbf^GMR.PD is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, osa^UAS.cCa

(Brumby et al., 2002)

NOT Suppressor of

Statement

Reference

Rbf^GMR.PD is a non-suppressor of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

Other

Statement

Reference

Rbf^GMR.PD, dap^GMR.PdN has eye disc & S phase phenotype

(Weng et al., 2003)

Nos^GMR.PK, Rbf^GMR.PD has pigment cell phenotype

(Kuzin et al., 2000)

Nos^GMR.PK, Rbf^GMR.PD has eye phenotype

(Kuzin et al., 2000)

Nos^GMR.PK, Rbf^GMR.PD has ommatidium phenotype

(Kuzin et al., 2000)

Nos^GMR.PK, Rbf^GMR.PD has interommatidial bristle phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has pigment cell phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has eye phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has ommatidium phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has interommatidial bristle phenotype

(Kuzin et al., 2000)

Rbf^GMR.PD, dap^GMR.PdN has eye phenotype

(de Nooij et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Rbf^GMR.PD when combined with dap^GMR.PdN can inhibit or delay S phase entry during the second mitotic wave in developing eye disc. The introduction of E2f^su89 restores normal S phase entry in the second wave. dap^GMR.PdN enhances the eye phenotype seen in dap^GMR.PdN animals. These phenotypes are also suppressed by the addition of E2f^su89.

(Weng et al., 2003)

Rbf^GMR.PD, Nos^hs.PK flies, treated with heatshock before pupariation, lead to adults with multiple defects in the eye, including missing bristles and pigment cells. A lack of the regular number of pigment cells in these flies results in the appearance of many fused ommatidia and a rough eye phenotype. These phenotypes are seen in flies with to copies of the transposon responsible for Rbf^GMR.PD (P{GMR-Rbf}) and become more severe when 4 copies of P{GMR-Rbf} are present. Rbf^GMR.PD, Nos^GMR.PK flies have missing pigment and bristle cells and fused ommatidia. The addition of BacA\p35^GMR.PH suppresses to wild-type the eye phenotype seen in E2f^GMR.PD, Dp^GMR.PD, Rbf^GMR.PD flies. The inhibition of Nos activity with L-NAME prevents this suppression. The arrangement of ommatidia is still abnormal, and many additional pigment cells are still observed.

(Kuzin et al., 2000)

Rbf^GMR.PD; CycE^JP double mutant flies have a more severe rough eye phenotype and greater reduction in the number of cells entering S phase posterior to the morphogenetic furrow than either Rbf^GMR.PD or CycE^JP single mutant flies.

(Secombe et al., 1998)

The mutant eye phenotype caused by Rbf^GMR.PD can be suppressed by coexpression of E2f^GMR.PD and Dp^GMR.PD. Rbf^GMR.PD expression in adults carrying two copies of E2f^GMR.PD and Dp^GMR.PD suppresses the rough eye phenotype, restoring the normal arrangement of ommatidia. CycE^AR95 heterozygotes carrying four copies of Rbf^GMR.PD exhibit eyes with fused ommatidia and missing bristles.

(Du et al., 1996)

Flies carrying a combination of dap^GMR.PdN and Rbf^GMR.PD exhibit extremely rough eyes.

(de Nooij et al., 1996)

Xenogenetic Interactions

Statement

Reference

The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is unaffected by Rbf^GMR.PD.

(Adamson et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rbf^GMR.PD

Name Synonyms

glass multimer reporter construct of Du

(Du et al., 1996)

Secondary FlyBase IDs

References (10)

Report Sections

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