FlyBase Allele Report: Dmel\dap[GMR.PdN]

General Information

Symbol

Dmel\dap^GMR.PdN

Species

D. melanogaster

Name

glass multimer reporter construct of de Nooij

FlyBase ID

FBal0057656

Feature type

allele

Associated gene

Dmel\dap

Associated Insertion(s)

Carried in Construct

P{GMR-dap}

Key Links

Transgenic product class

cDNA

(de Nooij et al., 1996)

wild_type

(de Nooij et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(de Nooij et al., 1996)

wild_type

(de Nooij et al., 1996)

Mutagen

in vitro construct

(Secombe et al., 1998, de Nooij et al., 1996)

Progenitor genotype

Carried in construct

P{GMR-dap}

Cytology

Description

dap cDNA fragment including the entire coding region and 27bp of 5' untranslated sequence and 193bp of 3' untranslated sequence is cloned into the GMR vector.

(de Nooij et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

dap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Staehling-Hampton et al., 1999, Secombe et al., 1998, de Nooij et al., 1996)

Phenotype Manifest In

eye

(Staehling-Hampton et al., 1999, Secombe et al., 1998)

ommatidium

(Staehling-Hampton et al., 1999)

Detailed Description

Statement

Reference

Expression of dap^GMR.PdN does not significantly effect BrdU incorporation in the second mitotic wave in the eye disc. Expression of dap^GMR.PdN results in no phenotype in the eye.

(Xin et al., 2002)

Flies expressing dap^GMR.PdN have a very mild rough eye phenotype.

(Secombe et al., 1998)

Flies carrying two copies of dap^GMR.PdN transgene appear wild type, normal complement of retinal cells. In flies bearing four copies of dap^GMR.PdN eyes are slightly rough, sections of these regions reveal missing pigment cells.

(de Nooij et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dap^GMR.PdN has visible phenotype, enhanceable by CycE^AR95

(Staehling-Hampton et al., 1999)

Suppressed by

Statement

Reference

Rbf^GMR.PD, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^280.GMR, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

CycE^AR95, dap^GMR.PdN has visible phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

Enhancer of

Statement

Reference

dap^GMR.PdN is an enhancer of decreased occurrence of cell division | larval stage phenotype of Rbf^280.GMR

(Xin et al., 2002)

dap^GMR.PdN is an enhancer of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

dap^GMR.PdN is a non-enhancer of visible phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

NOT Suppressor of

Statement

Reference

dap^GMR.PdN is a non-suppressor of visible phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

dap^GMR.PdN is a non-suppressor of abnormal size | adult stage phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

Other

Statement

Reference

Rbf^280.GMR, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype

(Weng et al., 2003)

Rbf^GMR.PD, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype

(Weng et al., 2003)

Rbf^GMR.PD, dap^GMR.PdN has visible phenotype

(de Nooij et al., 1996)

CycE^AR95, dap^GMR.PdN has visible phenotype

(de Nooij et al., 1996)

Phenotype Manifest In

Enhanced by

Statement

Reference

dap^GMR.PdN has eye phenotype, enhanceable by CycE^AR95

(Staehling-Hampton et al., 1999)

dap^GMR.PdN has ommatidium phenotype, enhanceable by CycE^AR95

(Staehling-Hampton et al., 1999)

Suppressed by

Statement

Reference

Rbf^GMR.PD, dap^GMR.PdN has eye disc & S phase phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^280.GMR, dap^GMR.PdN has eye disc & S phase phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

CycE^AR95, dap^GMR.PdN has eye phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

CycE^AR95, dap^GMR.PdN has ommatidium phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

Enhancer of

Statement

Reference

dap^GMR.PdN is an enhancer of interommatidial bristle phenotype of Rbf^GMR.PD

(Weng et al., 2003)

dap^GMR.PdN is an enhancer of ommatidium phenotype of Rbf^GMR.PD

(Weng et al., 2003)

dap^GMR.PdN is an enhancer of phenotype of Rbf^280.GMR

(Xin et al., 2002)

dap^GMR.PdN is an enhancer of phenotype of Rbf^GMR.PD

(Xin et al., 2002)

dap^GMR.PdN is an enhancer of eye phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

dap^GMR.PdN is a non-enhancer of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

NOT Suppressor of

Statement

Reference

dap^GMR.PdN is a non-suppressor of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

Other

Statement

Reference

Rbf^280.GMR, dap^GMR.PdN has eye disc & S phase phenotype

(Weng et al., 2003)

Rbf^GMR.PD, dap^GMR.PdN has eye disc & S phase phenotype

(Weng et al., 2003)

Rbf^280.GMR, dap^GMR.PdN has eye disc phenotype

(Xin et al., 2002)

CycE^AR95, dap^GMR.PdN has eye phenotype

(de Nooij et al., 1996)

Rbf^GMR.PD, dap^GMR.PdN has eye phenotype

(de Nooij et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Rbf^280.GMR when combined with dap^GMR.PdN can inhibit or delay S phase entry during the second mitotic wave in developing eye disc. The introduction of E2f^su89 restores normal S phase entry in the second wave. dap^GMR.PdN enhances the eye phenotype seen in dap^GMR.PdN animals. These phenotypes are also suppressed by the addition of E2f^su89.

(Weng et al., 2003)

Co-expression of dap^GMR.PdN and Rbf^280.GMR completely inhibits BrdU incorporation in the second mitotic wave in the eye disc.

(Xin et al., 2002)

Flies expressing dap^GMR.PdN have a mild rough eye phenotype, which is enhanced in the presence of one copy of CycE^AR95. The dap^GMR.PdN CycE^AR95 phenotype is suppressed by spen^poc361 and eye morphology is restored.

(Staehling-Hampton et al., 1999)

Dominantly enhances the rough eye phenotype of CycE^JP homozygotes.

(Secombe et al., 1998)

Flies carrying a combination of dap^GMR.PdN and Rbf^GMR.PD exhibit extremely rough eyes. Expression of dap^GMR.PdN transgene in CycE^AR95 flies causes a rough eye phenotype. Expression of dap^GMR.PdN transgene in CycA⁵, Df(2R)59AB or stg² flies has no effect on the eye phenotype.

(de Nooij et al., 1996)

Xenogenetic Interactions

Statement

Reference

The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is unaffected by dap^GMR.PdN.

(Adamson et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dap^GMR.PdN

Name Synonyms

glass multimer reporter construct of de Nooij

(de Nooij et al., 1996)

Secondary FlyBase IDs

References (6)

Report Sections

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