When wild-type females previously mated to wild-type males are mated with Tm1^gs1 males (which ejaculate seminal fluid but no sperm) the proportion of females with no stored sperm is significantly less than for females that have not undergone a second mating. However when the second mating is with prd⁴/Df(2L)Prl; prd^wt males (which do not ejaculate at all) the proportion of females with stored sperm is no different to that seen when the second mating is with Tm1^gs1 males, suggesting that seminal fluid is not necessary for the loss of stored sperm on mating.

Wild-type females mated to prd⁴/Df(2L)Prl males rescued by prd^wt extrude their ovipositors when subsequently challenged with wild-type males. The frequency of ovipositor extrusion elicited by the prd⁴/Df(2L)Prl males rescued by prd^wt is almost the same as that elicited by control males. However, wild-type females that had been mated to prd⁴/Df(2L)Prl males rescued by prd^wt and then challenged with wild-type males all remate within an hour, in contrast to wild-type females that had been mated to wild-type males and then challenged with wild-type males, which do not remate within an hour.

Females mated with sons of prd^wt; prd⁴/Df(2L)Prl mothers only show a partial stimulation of oviposition during the first day after mating and lay about 25% of the clutch induced by mating with wild-type males. This stimulation is enhanced slightly, but remains constant at 30-35% of oviposition induced by wild-type for at least 5 days. These females post copulation receptivity is indistinguishable from wild-type. When wild-type virgin females are mated with prd^wt; prd⁴/Df(2L)Prl males (who are normally sterile, lacking accessory glands) followed by tud¹ males (which are also normally sterile, lacking sperm) a few offspring are produced. However when this experiment is performed in reverse (copulation with tud¹ males first) no restoration of fertility is seen.