Construct: Start codon mutation of oskM1L is cloned as a 514bp BamHI-BssHII fragment into oskbcd.UTR.
Eggs produced by females carrying oskshort.bcd have ectopic pole cells at the anterior.
Expression in the anterior causes formation of bicaudal embryos that develop pole cells at their anterior.
oskshort.bcd has primordial germ cell | ectopic phenotype, suppressible by aubHN2/aubN11
Ectopic pole cells are not produced at the anterior of embryos derived from aubN11/aubHN ; oskshort.bcd females.