FlyBase Allele Report: Dmel\spi[SC1]

General Information

Symbol

Dmel\spi^SC1

Species

D. melanogaster

Name

FlyBase ID

FBal0056122

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Tio et al., 1994)

Mutagen

ethyl methanesulfonate

(Tio et al., 1994)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage | somatic clone

(Baker and Yu, 2001)

lethal | recessive

(Tio et al., 1994)

Phenotype Manifest In

Detailed Description

Statement

Reference

Photoreceptor differentiation is defective in somatic clones of spi^SC1 homozygous cells in the eye disc. This phenotype is rescued if the clones are made in a spi^Scer\UAS.cSa; Scer\GAL4^αTub84B.PL; Scer\GAL80^αTub84B.PL background and lack Scer\GAL80^αTub84B.PL. However, this rescue is not seen if spi^{CS.m.Scer\UAS} is used in place of spi^Scer\UAS.cSa.

(Miura et al., 2006)

Homozygous clones in the eye disc undergo less than half as many mitoses as control clones. Dividing cells within the clone are usually next to the boundary with wild-type cells. BrdU incorporation is normal in mutant cells.

(Baker and Yu, 2001)

Heterozygotes have wild-type wings.

(Nagaraj et al., 1999)

Germline clones reveal no requirement for spi in patterning the egg, or in the viability or patterning of the embryo.

(Wasserman and Freeman, 1998)

In mutant eye clones the initial single neuron stage ommatidia appear normal, as do their spacing. However no further progression occurs, and the clusters appear to be arrested at the one neuron stage. The wave of DNA synthesis immediately following the furrow is unaffected in spi mutant clones. The early development of the preclusters is normal up until the 5-cell stage. Development proceeds normally anterior to and in the furrow (up to the specification and early differentiation of the founding R8 photoreceptor cells) but the specification of R2 and R5, and all subsequent steps, fail. Homozygous spi mutant clones are apparently equal in size to their homozygous wild-type twin-spots.

(Tio and Moses, 1997)

Retinal clones induced during first larval instar show defects in the formation of photoreceptor cells R8, R2, R5, R3 and R4.

(Tio et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(2R)CX1, spi^SC1 has visible | dominant phenotype

(Nagaraj et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

spi^SC1 is an enhancer of phenotype of Egfr^E1

(Tio et al., 1994)

Suppressor of

Statement

Reference

spi[+]/spi^SC1 is a suppressor of phenotype of Src42A^Su(Raf)1-1

(Zhang et al., 1999)

Other

Statement

Reference

Df(3L)γ3, spi^SC1 has photoreceptor cell R3 | somatic clone phenotype

(Yang and Baker, 2003)

Df(3L)γ3, spi^SC1 has photoreceptor cell R2 | somatic clone phenotype

(Yang and Baker, 2003)

Df(3L)γ3, spi^SC1 has photoreceptor cell R4 | somatic clone phenotype

(Yang and Baker, 2003)

Df(3L)γ3, spi^SC1 has photoreceptor cell R5 | somatic clone phenotype

(Yang and Baker, 2003)

Df(3L)γ3, spi^SC1 has photoreceptor cell R8 | somatic clone phenotype

(Yang and Baker, 2001)

Df(2R)CX1, spi^SC1 has wing phenotype

(Nagaraj et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

In spi^SC1; Df(3L)γ3 double mutant somatic clones in the 3rd instar eye disc, photoreceptors R2-R5 undergo G1 arrest, but do not differentiate.

(Yang and Baker, 2003)

spi^SC1 ; Df(3L)γ3; double mutant clones in the eye disc develop similarly to spi^SC1 single mutant cells, but occasionally R8 spacing is affected so that twinned R8 cells are seen.

(Yang and Baker, 2001)

S^104E Df(2R)CX1 double heterozygotes show notching of the wing margin.

(Nagaraj et al., 1999)

Dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies.

(Zhang et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

spi^133a

(Faucheux et al., 2001)

Rescued by

spi¹/spi^SC1 is rescued by Scer\GAL4^da.G32/spi^UAS.cSa

(Miura et al., 2006)

Not rescued by

spi¹/spi^SC1 is not rescued by spi^CS.m.UAS/Scer\GAL4^da.G32

(Miura et al., 2006)

Comments

Unlike spi^Scer\UAS.cSa; Scer\GAL4^da.G32, spi^{CS.m.Scer\UAS}; Scer\GAL4^da.G32 completely fails to rescue lethality due to spi¹/spi^SC1.

(Miura et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

spi^SC1

(Mangione et al., 2023, Newcomb et al., 2018, Steinhauer et al., 2013)

spi^SC

(Faucheux et al., 2001)

spi^SE1

(Szuts et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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