FlyBase Allele Report: Dmel\norpA[ninaE.PM]

General Information

Symbol

Dmel\norpA^ninaE.PM

Species

D. melanogaster

Name

neither inactivation nor afterpotential E promoter construct of McKay

FlyBase ID

FBal0056441

Feature type

allele

Associated gene

Dmel\norpA

Associated Insertion(s)

Carried in Construct

P{ninaE/norpA}

Also Known As

rh1-norpA

Key Links

Transgenic product class

cDNA

(McKay et al., 1995)

wild_type

(McKay et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(McKay et al., 1995)

wild_type

(McKay et al., 1995)

Mutagen

in vitro construct

(Kim et al., 2003, van Huizen et al., 1998, Pearn et al., 1996, McKay et al., 1995)

Progenitor genotype

Carried in construct

P{ninaE/norpA}

Cytology

Description

A ninaE promoter drives expression of the norpA subtype I isoform.

(Kim et al., 2003)

Full length norpA is expressed under the control of a ninaE promoter.

(van Huizen et al., 1998)

norpA cDNA sequences from 519 to 4331 (coordinates according to FBrf0047660) are expressed under the control of a ninaE promoter.

(McKay et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

ninaE

Encoded product / tool

norpA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(van Huizen et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ninaE¹⁷, norpA^P24, norpA^ninaE.PM has abnormal neurophysiology | adult stage phenotype, suppressible | partially by ninaE^E194Q

(Zheng et al., 2015)

ninaE¹⁷, norpA^P24, norpA^ninaE.PM has abnormal neurophysiology | adult stage phenotype, suppressible by ninaE^D124N

(Zheng et al., 2015)

ninaE¹⁷, norpA^P24, norpA^ninaE.PM has abnormal neurophysiology | adult stage phenotype, suppressible | partially by ninaE^D96N

(Zheng et al., 2015)

ninaE¹⁷, norpA^P24, norpA^ninaE.PM has abnormal neurophysiology | adult stage phenotype, suppressible by ninaE^D203N

(Zheng et al., 2015)

ninaE¹⁷, norpA^P24, norpA^ninaE.PM has abnormal neurophysiology | adult stage phenotype, suppressible by ninaE^D147N

(Zheng et al., 2015)

Other

Statement

Reference

ninaE¹⁷, norpA^P24, norpA^ninaE.PM has abnormal neurophysiology | adult stage phenotype

(Zheng et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing norpA^ninaE.PM in norpA³⁶ ninaE¹⁷/ninaE¹⁷ background exhibit a dramatically reduced response to light (lack of a light induced depolarization and on and off transients) than controls in electroretinogram readings although they are stimulated with higher light intensity than controls.

(Zheng et al., 2015)

norpA³⁶ ; ninaE¹⁷/+ flies that carry norpA^ninaE.PM have a robust response to light at both 350nm and 520nm (measured using an electroretinogram recording). norpA³⁶ ; ninaE¹⁷/ninaE¹⁷ flies that carry norpA^ninaE.PM have no response to light at both 350nm and 520nm (measured using an ERG recording). Introduction of Lpol\Ops1^ninaE.PK or Lpol\Ops1^{ninaE.T:Btau\1D4} into a norpA³⁶ ; ninaE¹⁷/ninaE¹⁷ ; norpA^ninaE.PM background restores the response to light (measured using an ERG recording), although the response at 520nm is much great than that at 350nm.

(Knox et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

norpA^ninaE.PM rescues norpA^P24

(Kim et al., 2003)

norpA^ninaE.PM rescues norpA^P24

(van Huizen et al., 1998)

Partially rescues

norpA^ninaE.PM partially rescues norpA^unspecified

(Wernet et al., 2012)

norpA^ninaE.PM partially rescues norpA^P24

(Pearn et al., 1996)

norpA^ninaE.PM partially rescues norpA^P24

(McKay et al., 1995)

Comments

norpA^ninaE.PM fails to rescue a polarotactic response to linearly polarised UV light presented dorsally in norpA^unspecified flies.

norpA^ninaE.PM weakly rescues a polarotactic response to linearly polarised UV light presented ventrally in norpA^unspecified flies.

norpA^ninaE.PM rescues a polarotactic response to linearly polarised green light presented ventrally in norpA^unspecified flies.

(Wernet et al., 2012)

norpA^ninaE.PM flies are able to rescue the physiological defects in norpA³⁶ flies; the rescued flies exhibit electroretinograms (ERGs) that are similar in magnitude and shape to those of control flies.

(Kim et al., 2003)

norpA^ninaE.PM rescues the electrophysiological phenotype of norpA³⁶ flies in photoreceptor cells R1 to R6, but not in R7 and R8. The degeneration phenotype of the R1 to R6 photoreceptors is also rescued.

(Pearn et al., 1996)

Partially rescues the reduction in phospholipase C activity seen in the heads of norpA³⁶ flies. Also restores the electroretinogram and prolonged depolarising afterpotential responses of norpA³⁶ flies to a more normal response.

(McKay et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

norpA^ninaE.PM

Name Synonyms

neither inactivation nor afterpotential E promoter construct of McKay

(McKay et al., 1995)

Secondary FlyBase IDs

References (14)

Report Sections

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