FlyBase Allele Report: Dmel\Egm[1]

General Information

Symbol

Dmel\Egm¹

Species

D. melanogaster

Name

FlyBase ID

FBal0057535

Feature type

allele

Associated gene

Dmel\Egm

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Mourikis, 2006.3.2)

Mutagen

Progenitor genotype

Cytology

Description

Egm¹ contains a deletion corresponding to -424 to +241, relative to the starting ATG.

(Mourikis et al., 2006)

Deletion at the 5' end of the gene, deleting -423bp to +241bp relative to the starting ATG.

(Mourikis, 2006.3.2)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Mourikis et al., 2006)

lethal - all die before end of larval stage

(Mourikis, 2006.3.2, Mourikis et al., 2006)

long lived | dominant

(Mourikis et al., 2006)

some die during larval stage

(Mourikis, 2006.3.2)

some die during third instar larval stage

(Mourikis et al., 2006)

Phenotype Manifest In

embryonic/larval fat body

(Mourikis et al., 2006)

eye disc

(Mourikis et al., 2006)

wing disc

(Mourikis et al., 2006)

Detailed Description

Statement

Reference

Homozygous Egm¹ mutants die during the third instar larval stage. Although embryonic development seems normal, mutant Egm¹ animals enter a prolonged larval lifespan this is extended, on average, by 3.9-fold compared with wild-type. Late Egm¹ larvae develop sub-cuticular melanotic masses, have abnormally small eye and wing imaginal discs, and malformed fat bodies. The level of triglycerides is almost halved in Egm¹ larvae, while the transcription levels of Lip3 are highly up-regulated.

Egm¹ heterozygotes exhibit a wild-type developmental rate.

Egm¹ mutant clones exhibit aberrant morphology, in the adult eye retina, thoracic bristles, as well as the wing margin. Mutant ovarioles do not produce viable eggs.

At 25^oC, the median survival of female flies heterozygous for Egm¹ is on average 19.5% greater than in control flies.

(Mourikis et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Egm¹ is an enhancer of phenotype of N^nucl.sev

(Verheyen et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Second site dominant enhancer of N^nucl.sev. Eye phenotype is difficult to classify as the eye is larger and fuller, but appears more glossy than N^nucl.sev eyes.

(Verheyen et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Egm¹ is rescued by Egm^+tMa

(Mourikis et al., 2006)

Comments

Expression of the Egm^+tMa transgene rescues the lethality of Egm¹ flies.

(Mourikis et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

All 31 'alleles' referred in Table 2 of FBrf0090844 bear an identical molecular lesion, confirming that they are all members of a cluster, now represented by the single allele record - Egm¹.

(Mourikis, 2006.3.2)

FlyBase curator comment: FBrf0090844 described 31 alleles of Egm, generated by ethyl methanesulfonate or X ray mutagenesis. Subsequent work (Mourikis, 2006.3.1, personal communication to FlyBase) showed these alleles to be a cluster - now represented by Egm¹.

(Verheyen et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

Egm¹

(Mourikis et al., 2006, Mourikis et al., 2006)

egm^eAB6

(Verheyen et al., 1996)

egm^eAI6

(Verheyen et al., 1996)

egm^eAM2

(Verheyen et al., 1996)

egm^eAO5

(Verheyen et al., 1996)

egm^eB3

(Verheyen et al., 1996)

egm^eBS4

(Verheyen et al., 1996)

egm^eCN3

(Verheyen et al., 1996)

egm^eCO8

(Verheyen et al., 1996)

egm^eCP6

(Verheyen et al., 1996)

egm^eCR2

(Verheyen et al., 1996)

egm^eCS19

(Verheyen et al., 1996)

egm^eCS3

(Verheyen et al., 1996)

egm^eCU1

(Verheyen et al., 1996)

egm^eCW3

(Verheyen et al., 1996)

egm^eD6a

(Verheyen et al., 1996)

egm^eDN1

(Verheyen et al., 1996)

egm^eDY2

(Verheyen et al., 1996)

egm^sAF1

(Verheyen et al., 1996)

egm^sAH1

(Verheyen et al., 1996)

egm^sAH7

(Verheyen et al., 1996)

egm^sCS8

(Verheyen et al., 1996)

egm^sDC9

(Verheyen et al., 1996)

egm^sDP4

(Verheyen et al., 1996)

egm^sDZ1

(Verheyen et al., 1996)

egm^sEH1

(Verheyen et al., 1996)

egm^sEL1

(Verheyen et al., 1996)

egm^sF2a

(Verheyen et al., 1996)

egm^sI2

(Verheyen et al., 1996)

egm^sL7

(Verheyen et al., 1996)

egm^sP2

(Verheyen et al., 1996)

egm^sY3

(Verheyen et al., 1996)

Name Synonyms

Secondary FlyBase IDs

FBal0057505
FBal0057506
FBal0057507
FBal0057508
FBal0057509
FBal0057510
FBal0057511
FBal0057512
FBal0057513
FBal0057514
FBal0057515
FBal0057516
FBal0057517
FBal0057518
FBal0057519
FBal0057520
FBal0057521
FBal0057522
FBal0057523
FBal0057524
FBal0057525
FBal0057526
FBal0057527
FBal0057528
FBal0057529
FBal0057530
FBal0057531
FBal0057532
FBal0057533
FBal0057534

References (4)

Report Sections

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