Wild-type arm, expressed under the control of its own promoter. The protein is tagged with a Tag:MYC epitope at residue 784 in the Gly/Pro-rich region close to the C-terminus. arm 3' trailer sequences are also present. The protein produced shows normal levels of binding to α-Cat protein and to shg protein in a coimmunoprecipitation assay.
armS2.Tag:MYC rescues armunspecified
armS2.Tag:MYC rescues arm4
Rescues to adult viability and fertility.
Lethality is rescued to adulthood.
Used in a coimmunoprecipitation assay to study shg binding and analysed by SDS-PAGE to study protein phosphorylation and stability.