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General Information
Symbol
Dmel\RafDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0059973
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-RafDN
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a dominant negative form of Raf.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing RafDN.UAS under the control of Scer\GAL4Bx-MS1096 results in a reduced wing size and loss of vein material.

Migration of many of the ganglionic tracheal branches is stalled or misrouted in phlDN.Scer\UAS; Scer\GAL4bs-23.26 embryos, but branches do not cross the ventral midline.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The wing defects induced by the expression of RafDN.UAS under the control of Scer\GAL4Bx-MS1096 (smaller wing and loss of L3-5 vein material are: fully suppressed by the co-expression of either rlD334N.UAS.cKa or rlR80S.D334N.UAS, even leading to extra vein material (more severe in the latter, with darker pigmentation); and partially suppressed by the co-expression of rlR80S.UAS or rlWT.UAS (partial rescue of the proximal part of L3 and distal part of L4, or complete rescue of L5).

Reduces but does not completely suppress the ectopic wing vein formation seen in tkvQ253D.Scer\UAS.cLa Scer\GAL4bs-1348 flies, when expressed using Scer\GAL4bs-1348.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
RafDN.Scer\UAS
RafDN.UAS
phlDN.Scer\UAS
phlDN.UAS
Name Synonyms
Secondary FlyBase IDs
    References (5)